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Merck
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主要文書

安全性情報

09-843

Sigma-Aldrich

Anti-SIRT2 Antibody

from rabbit, purified by affinity chromatography

別名:

SIR2-like protein 2, silencing information regulator 2-like, silent information regulator 2, sir2-related protein type 2, sirtuin (silent mating type information regulation 2, homolog) 2 (S. cerevisiae)

sirtuin (silent mating type information r

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About This Item

UNSPSCコード:
12352203
eCl@ss:
32160702
NACRES:
NA.41

由来生物

rabbit

品質水準

抗体製品の状態

affinity isolated antibody

抗体製品タイプ

primary antibodies

クローン

polyclonal

精製方法

affinity chromatography

交差性

human, mouse

テクニック

immunoprecipitation (IP): suitable
western blot: suitable

NCBIアクセッション番号

UniProtアクセッション番号

輸送温度

wet ice

ターゲットの翻訳後修飾

unmodified

遺伝子情報

human ... SIRT2(22933)

詳細

Sirtuins are hypothesized to play a key role in an organism′s response to stresses (such as heat or starvation) and to be responsible for the lifespan-extending effects of calorie restriction. Sirtuins act by removing acetyl groups from proteins in the presence of NAD+; they are thus classified as "NAD+-dependent deacetylases". Mammals have seven sirtuins; SIRT1 and SIRT2 occupy the cytoplasm. SIRT2 is a NAD-dependent deacytylase. It has been shown to control gene silencing, cell cycle, and DNA damage repair. In additon, SIRT2 acts as a tumor suppressor in human gliomas which suggests that the ectopic expression of SIRT2 upsets the microtubule network.

特異性

This antibody recognizes the mature protein of SIRT2.

免疫原

Epitope: Mature protein
KLH-conjugated linear peptide corresponding to the mature protein of human SIRT2.

アプリケーション

Research Category
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン修飾タンパク質
Use Anti-SIRT2 Antibody (Rabbit Polyclonal Antibody) validated in WB, IP to detect SIRT2 also known as SIR2-like protein 2, silent information regulator 2, sirtuin 2.
Western Blot (SNAP ID) Analysis: 0.25 µg/mL from a representative lot detected SIRT2 on 10 µg of human brain tissue lysate.

Immunoprecipitation Analysis: 5 µg from a representative lot immunoprecipitated SIRT2 in human brain lysate.

品質

Evaluated by Western Blot in human brain tissue lysate.

Western Blot Analysis: 0.125 µg/mL of this antibody detected SIRT2 on 10 µg of human brain tissue lysate.

ターゲットの説明

Observed molecular weight is ~39 kDa Multiple isoforms around 40 kDa may be observed.

関連事項

Replaces: 04-1124

物理的形状

Affinity purified
Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

保管および安定性

Stable for 1 year at 2-8°C from date of receipt.

アナリシスノート

Control
Human brain tissue lysate

その他情報

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

免責事項

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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保管分類コード

12 - Non Combustible Liquids

WGK

WGK 1

引火点(°F)

Not applicable

引火点(℃)

Not applicable


適用法令

試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。

Jan Code

09-843:


試験成績書(COA)

製品のロット番号・バッチ番号を入力して、試験成績書(COA) を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。

以前この製品を購入いただいたことがある場合

文書ライブラリで、最近購入した製品の文書を検索できます。

文書ライブラリにアクセスする

Jonathan P Belman et al.
The Journal of biological chemistry, 290(7), 4447-4463 (2015-01-07)
Insulin causes the exocytic translocation of GLUT4 glucose transporters to stimulate glucose uptake in fat and muscle. Previous results support a model in which TUG traps GLUT4 in intracellular, insulin-responsive vesicles termed GLUT4 storage vesicles (GSVs). Insulin triggers TUG cleavage
Kiril Tuntevski et al.
FASEB bioAdvances, 2(7), 387-397 (2020-07-18)
Stroke causes severe long-term disability in patients due to the induction of skeletal muscle atrophy and weakness, but the molecular mechanisms remain elusive. Using a preclinical mouse model of cerebral ischemic stroke, we show that stroke robustly induced atrophy and
Sung Eun Wang et al.
Experimental neurobiology, 28(4), 537-546 (2019-09-09)
Silent information regulator 2 (Sirtuin2 / SIRT2) is a NAD+-dependent deacetylase that regulates the cellular oxidative stress response. It modulates transcriptional silencing and protein stability through deacetylation of target proteins including histones. Previous studies have shown that SIRT2 plays a
Elizabeth V Minten et al.
Cell reports, 34(13), 108921-108921 (2021-04-01)
The breast cancer type I susceptibility protein (BRCA1) and BRCA1-associated RING domain protein I (BARD1) heterodimer promote genome integrity through pleiotropic functions, including DNA double-strand break (DSB) repair by homologous recombination (HR). BRCA1-BARD1 heterodimerization is required for their mutual stability

ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.

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