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由来生物
rabbit
品質水準
抗体製品の状態
culture supernatant
抗体製品タイプ
primary antibodies
クローン
62-141-13, monoclonal
交差性
Saccharomyces cerevisiae, human, vertebrates, chicken
メーカー／製品名
Upstate®
テクニック
ChIP: suitable
multiplexing: suitable
western blot: suitable
アイソタイプ
IgG
NCBIアクセッション番号
UniProtアクセッション番号
輸送温度
dry ice
ターゲットの翻訳後修飾
unmodified
遺伝子情報
human ... H4C1(8359)
詳細
This antibody recognizes histone H4 independant of any post-translational modifications.
特異性
Recognizes Histone H4.
免疫原
Epitope: a.a. 25-28
Synthetic peptide corresponding to amino acids 17-28 of Histone H4. Epitope has been mapped to residues 25-28
アプリケーション
Research Category
エピジェネティクス及び核内機能分子
エピジェネティクス及び核内機能分子
Research Sub Category
ヒストン
ヒストン
Anti-Histone H4 Antibody, pan is a Rabbit Monoclonal Antibody for detection of Histone H4 also known as Histone H4 & has been validated in ChIP, WB, Mplex.
Immunoblot Analysis:
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
A 1:100,000 dilution of a previous lot detected unmodified recombinant Histone H4 protein (Catalog # 14-412) and Histone H4 in acid extracts from both untreated HeLa cells and cells treated with sodium butyrate (hyper-acetylated) or colcemid (hyper-phosphorylated) (Figure B).
Chromatin Immunoprecipitation:
An independent laboratory used a previous lot of this antibody in chromatin immunoprecipitation (Issac, C., 2006).
Multiplexing:
Serial dilutions of a previous lot were incubated with histone peptides containing various unmodified peptides spanning the aminoterminus of Histone H4 conjugated to Luminex® microspheres. The antibody recognized both modified and unmodified peptides.
品質
Routinely evaluated by immunoblot using HeLa cell acid extract.
ターゲットの説明
10 kDa
関連事項
Replaces: 05-858
物理的形状
Cultured supernantant in 0.05% sodium azide.
保管および安定性
Stable for 1 year at -20°C from date of receipt.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
For maximum recovery of product, centrifuge the vial prior to removing the cap. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
アナリシスノート
Control
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.
Recombinant Histone H4 (Catalog # 14-412) or HeLa cell acid extract.
法的情報
Luminex is a registered trademark of Luminex Corp
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
免責事項
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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保管分類コード
12 - Non Combustible Liquids
WGK
WGK 1
引火点(°F)
Not applicable
引火点(℃)
Not applicable
適用法令
試験研究用途を考慮した関連法令を主に挙げております。化学物質以外については、一部の情報のみ提供しています。 製品を安全かつ合法的に使用することは、使用者の義務です。最新情報により修正される場合があります。WEBの反映には時間を要することがあるため、適宜SDSをご参照ください。
Jan Code
04-858:
試験成績書（COA）
製品のロット番号・バッチ番号を入力して、試験成績書（COA） を検索できます。ロット番号・バッチ番号は、製品ラベルに「Lot」または「Batch」に続いて記載されています。
Multiple forms of H4 histone mRNA in human cells.
Lichtler, A C, et al.
Proceedings of the National Academy of Sciences of the USA, 77, 1942-1946 (1980)
Soheila Dolatabadi et al.
Frontiers in oncology, 12, 816894-816894 (2022-02-22)
Myxoid liposarcoma is one of the most common sarcoma entities characterized by FET fusion oncogenes. Despite a generally favorable prognosis of myxoid liposarcoma, chemotherapy resistance remains a clinical problem. This cancer stem cell property is associated with JAK-STAT signaling, but
Peter J Thompson et al.
PLoS genetics, 11(1), e1004933-e1004933 (2015-01-23)
Retrotransposition of endogenous retroviruses (ERVs) poses a substantial threat to genome stability. Transcriptional silencing of a subset of these parasitic elements in early mouse embryonic and germ cell development is dependent upon the lysine methyltransferase SETDB1, which deposits H3K9 trimethylation
Venizelos Papayannopoulos et al.
The Journal of cell biology, 191(3), 677-691 (2010-10-27)
Neutrophils release decondensed chromatin termed neutrophil extracellular traps (NETs) to trap and kill pathogens extracellularly. Reactive oxygen species are required to initiate NET formation but the downstream molecular mechanism is unknown. We show that upon activation, neutrophil elastase (NE) escapes
Ryan W Logan et al.
Molecular psychiatry, 26(8), 4066-4084 (2020-11-26)
Valproate (VPA) has been used in the treatment of bipolar disorder since the 1990s. However, the therapeutic targets of VPA have remained elusive. Here we employ a preclinical model to identify the therapeutic targets of VPA. We find compounds that
ライフサイエンス、有機合成、材料科学、クロマトグラフィー、分析など、あらゆる分野の研究に経験のあるメンバーがおります。.
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