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A8111

Sigma-Aldrich

Plasminogen activator inhibitor 1 (PAI-1)

≥200,000 units/mg protein, lyophilized powder, ≥90% (SDS-PAGE)

Synonym(s):

PAIE, PLANH1, Plasminogen activator inhibitor, β-migrating endothelial cell-derived type, Serpin E1 PAI-1, Serpine 1

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About This Item

CAS Number:
140208-23-7
MDL number:
MFCD01094930
UNSPSC Code:
12352200
NACRES:
NA.32

product name

Plasminogen activator inhibitor 1 (PAI-1) human, recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

recombinant

expressed in E. coli

Quality Level

200

Assay

≥90% (SDS-PAGE)

form

lyophilized powder

specific activity

≥200,000 units/mg protein

mol wt

~43 kDa

solubility

water: 0.1 mL, clear, colorless

UniProt accession no.

P05121

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... SERPINE1(5054)

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General description

Plasminogen activator inhibitor 1 (PAI-1) or serine protease inhibitor E1 (SERPINE1) gene is mapped to human chromosome 7q22.1. PAI-1 is produced in hepatocytes, endothelial cells, smooth muscle cells, megakaryocytes, and adipocytes.

Application

Plasminogen activator inhibitor 1 (PAI-1) human has been used:
  • as an inhibitor of pro-brain-derived neurotrophic factor (proBDNF) in radioimmunoprecipitation assay (RIPA) in peripheral blood and lymphocytes
  • to block plasmin activation in mice infected with C. albicans hyphae
  • to test its effect on neurite growth in neurons

Biochem/physiol Actions

Plasminogen Activator Inhibitor 1 (PAI-1) protein belongs to the Serpin superfamily of serine protease inhibitors. It is the principal inhibitor of tissue plasminogen activator (tPA) and urokinase (uPA), the activators of plasminogen and therefore inhibits the fibrinolysis process. PAI is involved in extracellular matrix remodeling and has been implicated in processes like angiogenesis, chemotaxis, ovulation, and embryogenesis. PAI-1 is present in increased levels in various disease states such as a number of cancer forms, obesity and the metabolic syndrome.
Plasminogen activator inhibitor–1 (PAI -1) participates in mediating cellular senescence. It is upregulated during injury along with other tissue repair transcriptomes. Elevated levels of PAI-1 is regarded as one of the risks associated with myocardial infarction (MI).

Unit Definition

1 unit irreversibly inhibits (100% inhibition) 1 unit of tissue plasminogen activator (tPA) under physiological conditions (neutral pH and 37 ºC).

Physical form

Supplied as a lyophilized powder from a solution containing 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Reconstitution

Reconstitute to 0.25 mg/ml with ultrapure water. The reconstituted product contains 0.25 mgP/mL in 0.5 M sodium chloride, 20 mM sodium acetate, and 50 mg/mL trehalose.

Analysis Note

The product is the active human PAI-1 protein with a molecular weight of 43 kDa.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A8111-25UG-PW:
A8111-VAR:
A8111-BULK:
A8111-25UG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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William Santus et al.
Science immunology, 2(15) (2017-09-25)
Nuclear factor of activated T cells (NFAT) is activated in innate immune cells downstream of pattern recognition receptors, but little is known about NFAT's functions in innate immunity compared with adaptive immunity. We show that early activation of NFAT balances
Bo-Yang Yu et al.
Biology of reproduction, 100(6), 1473-1481 (2019-04-03)
Plasminogen activator, tissue type (PLAT) and its inhibitor serpin family E member 1 (SERPINE1) cooperatively regulate PLAT activity in various reproductive processes. However, it is unknown whether this includes bovine oocyte maturation. We addressed this question in the present study
Natália Salazar et al.
Research in microbiology, 168(2), 157-164 (2016-12-19)
A previous study had demonstrated that Leptospira enolase is secreted extracellularly by a yet unknown mechanism and reassociates with the bacterial membrane. Surface-anchored leptospiral enolase displays plasminogen binding activity. In this work, we explored the consequences of this interaction and
Winfried Neuhaus et al.
Frontiers in molecular neuroscience, 10, 149-149 (2017-06-13)
The blood-brain barrier (BBB) is damaged during ischemic insults such as traumatic brain injury or stroke. This contributes to vasogenic edema formation and deteriorate disease outcomes. Enormous efforts are pursued to understand underlying mechanisms of ischemic insults and develop novel

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