Passa al contenuto
Merck
  • Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels.

Toward structured macroporous hydrogel composites: electron beam-initiated polymerization of layered cryogels.

Biomacromolecules (2015-03-03)
Anna Golunova, David Chvátil, Pavel Krist, Josef Jaroš, Veronika Jurtíková, Jakub Pospíšil, Ilya Kotelnikov, Lucie Abelová, Jiří Kotek, Tomáš Sedlačík, Jan Kučka, Jana Koubková, Hana Studenovská, Libor Streit, Aleš Hampl, František Rypáček, Vladimír Proks
ABSTRACT

The ability to tailor mechanical properties and architecture is crucial in creating macroporous hydrogel scaffolds for tissue engineering. In the present work, a technique for the modification of the pore size and stiffness of acrylamide-based cryogels is demonstrated via the regulation of an electron beam irradiation dose. The samples were characterized by equilibrium swelling measurements, light and scanning electron microscopy, mercury porosimetry, Brunauer-Emmett-Teller surface area analysis, and stiffness measurements. Their properties were compared to cryogels prepared by a standard redox-initiated radical polymerization. A (125)I radiolabeled azidopentanoyl-GGGRGDSGGGY-NH2 peptide was bound to the surface to determine the concentration of the adhesive sites available for biomimetic modification. The functionality of the prepared substrates was evaluated by in vitro cultivation of adipose-derived stem cells. Moreover, the feasibility of preparing layered cryogels was demonstrated. This may be the key to the future preparation of complex hydrogel-based scaffolds to mimic the extracellular microenvironment in a wide range of applications.

MATERIALI
N° Catalogo
Marchio
Descrizione del prodotto

Sigma-Aldrich
Copper(II) sulfate pentahydrate, ACS reagent, ≥98.0%
Sigma-Aldrich
DAPI, for nucleic acid staining
Sigma-Aldrich
Acrylamide, suitable for electrophoresis, ≥99%
Sigma-Aldrich
(+)- L-ascorbato di sodio, crystalline, ≥98%
Sigma-Aldrich
Bromuro di etidio, BioReagent, for molecular biology, 10 mg/mL in H2O
Sigma-Aldrich
(+)- L-ascorbato di sodio, BioXtra, ≥99.0% (NT)
Sigma-Aldrich
(+)- L-ascorbato di sodio, powder, BioReagent, suitable for cell culture
Sigma-Aldrich
Acrylamide, for molecular biology, ≥99% (HPLC)
Sigma-Aldrich
N,N′-Methylenebisacrylamide, powder, for molecular biology, suitable for electrophoresis, ≥99.5%
Sigma-Aldrich
Copper(II) sulfate pentahydrate, BioReagent, suitable for cell culture, ≥98%
Sigma-Aldrich
Acrylamide solution, 40%, suitable for electrophoresis, sterile-filtered
Sigma-Aldrich
Ethidium bromide, BioReagent, for molecular biology, powder
Sigma-Aldrich
Bromuro di etidio, BioReagent, for molecular biology, 500 μg/mL in H2O
Sigma-Aldrich
Acrylamide, suitable for electrophoresis, ≥99% (HPLC), powder
Sigma-Aldrich
N,N′-Methylenebisacrylamide solution, suitable for electrophoresis, 2% in H2O
Sigma-Aldrich
N,N′-Methylenebis(acrylamide), 99%
Sigma-Aldrich
Glutaraldeide, Grade I, 25% in H2O, specially purified for use as an electron microscopy fixative
Sigma-Aldrich
Acridine Orange hemi(zinc chloride) salt, For nucleic acid staining in cells or gels
Sigma-Aldrich
Glutaraldeide, 50 wt. % in H2O
Sigma-Aldrich
Glutaraldeide, Grade II, 25% in H2O
Sigma-Aldrich
4′,6-diamidino-2-fenilindolo, suitable for fluorescence, BioReagent, ≥95.0% (HPLC)
Sigma-Aldrich
Copper(II) sulfate pentahydrate, suitable for plant cell culture, ≥98%
Sigma-Aldrich
Copper(II) sulfate pentahydrate, 99.999% trace metals basis
Sigma-Aldrich
Bromuro di etidio, for fluorescence, ~1% in H2O
Sigma-Aldrich
Glutaraldeide, 50 wt. % in H2O, FCC
Supelco
(+)- L-ascorbato di sodio, Pharmaceutical Secondary Standard; Certified Reference Material
Sigma-Aldrich
Copper(II) sulfate pentahydrate, 99.995% trace metals basis
Sigma-Aldrich
N,N′-Methylenebisacrylamide, suitable for electrophoresis (after filtration or allowing insolubles to settle)
Sigma-Aldrich
Glutaraldeide, Grade I, 70% in H2O, specially purified for use as an electron microscopy fixative or other sophisticated use
Sigma-Aldrich
Ethidium bromide, ~95% (HPLC)