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M7279

Sigma-Aldrich

N,N′-Methylenebisacrylamide

powder, for molecular biology, suitable for electrophoresis, ≥99.5%

Sinonimo/i:

BIS

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About This Item

Formula condensata:
(CH2=CHCONH)2CH2
Numero CAS:
Peso molecolare:
154.17
Beilstein:
1706297
Numero CE:
Numero MDL:
Codice UNSPSC:
41105319
ID PubChem:
NACRES:
NB.22

Grado

for molecular biology

Livello qualitativo

Saggio

≥99.5%

Stato

powder

tecniche

electrophoresis: suitable

Impurezze

≤0.05% Free carboxylic acid (T)
≤50 mg/kg Total sulfur (as SO4 ) (ICP)

Conducibilità

≤10 μmho (2% solution)

Anioni in tracce

chloride (Cl-): ≤50 mg/kg

Cationi in tracce

Ca: ≤10 ppm
Cd: ≤5 ppm
Co: ≤5 ppm
Cr: ≤5 ppm
Cu: ≤5 ppm
Fe: ≤5 ppm
K: ≤50 ppm
Mg: ≤5 ppm
Mn: ≤5 ppm
Na: ≤50 ppm
Ni: ≤5 ppm
Pb: ≤5 ppm
Zn: ≤5 ppm

Assorbimento

≤0.2 at 290 at 1%

Attività estranea

DNase and RNase, none detected

Stringa SMILE

C=CC(=O)NCNC(=O)C=C

InChI

1S/C7H10N2O2/c1-3-6(10)8-5-9-7(11)4-2/h3-4H,1-2,5H2,(H,8,10)(H,9,11)
ZIUHHBKFKCYYJD-UHFFFAOYSA-N

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Categorie correlate

Descrizione generale

N,N′-Methylenebisacrylamide is commonly called as bis. It is used as a cross-linking agent in the preparation of polyacrylamide gels consisting of chains of acrylamide monomers. The concentration of N,N′-methylenebisacrylamide and acrylamide determines the pore size of the gel. The higher concentration of bis forms rigid gels with smaller pores due to more cross-linking leading to polymerization and vice versa.

Applicazioni

N,N′-Methylenebisacrylamide has been used:
  • as a crosslinker in the monomer solution to prepare N,N-dimethyl acrylamide (DMAAm)-based cryogel and acrylamide (AAm)-based cryogel matrix
  • as a crosslinker to prepare tough hydrogel network
  • in the preparation of acrylamide-bisacrylamide stock solution for the preparation of polyacrylamide gel

To be mixed with acrylamide for making polyacrylamide gels for use in protein and nucleic acid electrophoresis.

Pittogrammi

Skull and crossbonesHealth hazard

Avvertenze

Danger

Indicazioni di pericolo

Classi di pericolo

Acute Tox. 3 Oral - Acute Tox. 4 Dermal - Carc. 1B - Muta. 1B - Repr. 2 - STOT RE 1 Oral

Organi bersaglio

Peripheral nervous system

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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