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Key Documents

Altri documenti

T7024

Sigma-Aldrich

N,N,N′,N′-tetrametil-etilendiammina

BioReagent, for molecular biology, ≥99% (GC)

Sinonimo/i:

1,2-bis(dimetilamino)etano, TEMED, TMEDA

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About This Item

Formula condensata:
(CH3)2NCH2CH2N(CH3)2
Numero CAS:
110-18-9
Peso molecolare:
116.20
Beilstein:
1732991
Numero CE:
203-744-6
Numero MDL:
MFCD00008335
Codice UNSPSC:
41105319
ID PubChem:
24900242
NACRES:
NA.31

Grado

for molecular biology

Nome Commerciale

BioReagent

Saggio

≥99% (GC)

Limite di esplosione

9.08 %

Indice di rifrazione

n20/D 1.4179 (lit.)

P. eboll.

120-122 °C (lit.)

Punto di fusione

−55 °C (lit.)

Densità

0.775 g/mL at 20 °C (lit.)

Attività estranea

DNase, RNase, protease, none detected

Stringa SMILE

CN(C)CCN(C)C

InChI

1S/C6H16N2/c1-7(2)5-6-8(3)4/h5-6H2,1-4H3
KWYHDKDOAIKMQN-UHFFFAOYSA-N

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Applicazioni

This product is designated as molecular biology grade and is suitable for polyacrylamide gel electrophoresis (PAGE). It has been analyzed for the absence of nucleases and proteases.
TEMED is a tertiary amine that is used to catalyze the formation of free radicals from ammonium persulfate or riboflavin. TEMED-generated free radicals cause the compounds acrylamide and bis-acrylamide to polymerize and form the gel matrix polyacrylamide. Because TEMED is functional only in its free base form, low pH conditions will inhibit polymerization.,,
The potential contribution of TEMED to elevated background in silver stained SDS PAGE has been studied. TEMED has been used in the coating of soft polymers, such as polyacrylamide, in capillaries for investigation by atomic force microscopy. Microchips containing oligonucleotides and proteins immobilized within gel pads have been prepared using TEMED.
The capillary isoelectric focusing of proteins with an isoelectric point (pI) > 12 has been investigated through the use of TEMED. TEMED has been used in the HPLC of small organic molecules.

Quantità

Suggested use is 35 μl for 50 ml acrylamide gel solution.

Avvertenze

Danger

Indicazioni di pericolo

H225,H302,H314,H331

Classi di pericolo

Acute Tox. 3 Inhalation - Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 2 - Skin Corr. 1B

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

61.7 °F - closed cup

Punto d’infiammabilità (°C)

16.5 °C - closed cup

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Ammonium persulfate ACS reagent, ≥98.0%

Sigma-Aldrich

248614

Ammonium persulfate

Acrylamide solution 40%, suitable for electrophoresis, sterile-filtered

Sigma-Aldrich

A4058

Acrylamide solution

Acrylamide suitable for electrophoresis, ≥99%

Sigma-Aldrich

A8887

Acrylamide

Acrylamide/Bis-acrylamide, 40% solution BioReagent, suitable for electrophoresis, 19:1

Sigma-Aldrich

A9926

Acrylamide/Bis-acrylamide, 40% solution

H Rosing et al.
Journal of chromatography. B, Biomedical applications, 668(1), 107-115 (1995-06-09)
A sensitive high-performance liquid chromatographic (HPLC) assay has been developed and validated for the quantitation of the novel anticancer agent topotecan and topotecan as the total of its lactone and carboxylate forms in human plasma. Linear response in analyte standard
G Patras et al.
Electrophoresis, 20(10), 2039-2045 (1999-08-18)
A novel experimental procedure to study the factors that contribute to background silver staining in sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) is described. A mechanism is proposed for background silver staining, which was found to result primarily from the redox
Deepa Mohan et al.
Journal of chromatography. A, 979(1-2), 271-276 (2002-12-25)
The non-availability of commercial carrier ampholytes in the pH range greater than 11 has contributed to difficulties in focusing and resolving highly basic proteins/peptides using capillary isoelectric focusing (cIEF). Two different approaches, involving the use of N,N,N',N'-tetramethylethylenediamine (TEMED) and ampholyte
R Barberi et al.
Journal of chromatography. B, Biomedical applications, 683(1), 3-13 (1996-08-09)
Atomic force microscopy (AFM) has been used to probe the surface of a capillary after coating with "soft" polymers, notably polyacrylamides. The aim was the investigation of the efficiency of coverage of the silica surface, so as to reduce or
Sambrook, J. and Russell, D. W.
Molecular Cloning: A Laboratory Manual, 5-5 (2001)
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