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Key Documents

807448

TOYOPEARL® HW-40F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 30-60 μm

Sinonimo/i:

TOYOPEARL® HW-40 Size Exclusion Media

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About This Item

Codice UNSPSC:
41115700

Descrizione

HW-40F

Nome Commerciale

TOYOPEARL®

Forma fisica

slurry

Confezionamento

bottle of 500 mL

Parametri

3 bar max. pressure

tecniche

HPLC: suitable

Matrice

hydroxylated methacrylic polymer

Gruppo funzionale matrice

hydroxylated methacrylate phase

Dimensione particelle

30-60 μm

Dimensione pori

100-7000 Da MW range

pH di lavoro

2-13

Tecnica di separazione

size exclusion (SEC)

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Descrizione generale

Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.

Applicazioni

Toyopearl media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Specifiche

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Stato fisico

Shipped in 20% (v/v) ethanol.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

Toyopearl is a registered trademark of Tosoh Corporation

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Classi di pericolo

Flam. Liq. 3

Classe di pericolosità dell'acqua (WGK)

WGK 1

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
107H0370
097H0647

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Francesca Magnani et al.
Nature protocols, 11(8), 1554-1571 (2016-07-29)
The thermostability of an integral membrane protein (MP) in detergent solution is a key parameter that dictates the likelihood of obtaining well-diffracting crystals that are suitable for structure determination. However, many mammalian MPs are too unstable for crystallization. We developed
M V Pokrovskaya et al.
Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
Shu-fen Xu et al.
Fitoterapia, 83(1), 153-160 (2011-11-01)
Persimmon proanthocyanidin was fractionated on Toyopearl TSK-HW-50-F to yield a fraction with strong inhibition on the catalytic activity and edema-inducing activity and lethality of Chinese cobra PLA(2). Thiolysis suggested that the terminal units included C, EGCG and myricetin, and epicatechin
Olga N Smol'kina et al.
Carbohydrate research, 347(1), 161-163 (2011-11-22)
A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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