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807465

TOYOPEARL® HW-65F Bulk Media

phase hydroxylated methacrylate, bottle of 500 mL, 30-60 μm particle size

Sinonimo/i:

TOYOPEARL® HW-65 Size Exclusion Media

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About This Item

Codice UNSPSC:
41115700

Descrizione

HW-65F

Nome Commerciale

TOYOPEARL®

Stato

slurry

Confezionamento

bottle of 500 mL

Parametri

3 bar max. pressure

tecniche

HPLC: suitable

Matrice

hydroxylated methacrylic polymer

Gruppo funzionale matrice

hydroxylated methacrylate phase

Dimensione particelle

30-60 μm

Dimensione pori

10,000-1,000,000 Da MW range

pH di lavoro

2-13

Tecnica di separazione

size exclusion (SEC)

Descrizione generale

Toyopearl HW-65 resin is a hydroxylated methacrylic polymer bead for size exclusion chromatography of proteins between 40,000-5,000,000 Da. It also is the base bead for all Toyopearl "-650" designated products.

Applicazioni

TOYOPEARL® media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.

Specifiche

Clean in place with 0.5 M NaOH or 0.1 M HCl.

Stato fisico

Shipped in 20% (v/v) ethanol.

Prodotti consigliati

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

Note legali

Toyopearl is a registered trademark of Tosoh Corporation

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Classi di pericolo

Flam. Liq. 3

Classe di pericolosità dell'acqua (WGK)

WGK 1

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number

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TOYOPEARL® HW-40F Bulk Media phase hydroxylated methacrylate, bottle of 500 mL, 30-60 μm

807448

TOYOPEARL® HW-40F Bulk Media

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Protein expression and purification, 82(1), 150-154 (2012-01-10)
We have cloned ansB (YPTB1411) gene from Yersinia pseudotuberculosis Q66CJ2 and constructed stable inducible expression system that overproduce L-asparaginase from Y. pseudotuberculosis (YpA) in Escherichiacoli BL21 (DE3) cells. For purification of YpA we used Q-Sepharose and DEAE-Toyopearl column chromatography. We
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Olga N Smol'kina et al.
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A method is developed for the preparation of D-rhamnose from an O-polysaccharide (OPS) isolated by mild acid hydrolysis of Azospirillum brasilense SR75 cell mass. After the OPS hydrolysis, D-rhamnose was recovered by gel-permeation chromatography on Toyopearl TSK HW-40 and was

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