G10120
Sephadex® G-10
Medium
Sinonimo/i:
Gel filtration resin, Sephadex G-10 Medium
Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
About This Item
Prodotti consigliati
Livello qualitativo
tecniche
protein array: suitable
Gruppo funzionale matrice
phase
Rigonfiamento
1 g swells to 2-3 mL
applicazioni
life science and biopharma
Compatibilità
Cytiva
Cerchi prodotti simili? Visita Guida al confronto tra prodotti
Descrizione generale
Sephadex G-10 is a well-established gel filtration resin specifically designed for desalting and buffer exchange of peptides and small biomolecules. It boasts the smallest exclusion limit within the Sephadex range, making it highly effective in these applications. Sephadex itself is a gel filtration resin that is created through the crosslinking of dextran with epichlorohydrin. The various types of Sephadex exhibit different degrees of cross-linking, leading to variations in swelling properties and molecular fractionation range. Sephadex G-10 falls into the category of G-types, alongside four other types, ranging from G-10 for small molecules to G-75 for larger molecules.
Applicazioni
Sephadex® G-10 has been used as a size exclusion material for the separation of the conjugated N-glycans from the surplus dye. It has also been used in desalting for reducing salt concentration for anion-exchange (AEx), cation-exchange (CEx) chromatography.
Sephadex® G-10 has been used for the reconstitution of membrane proteins into a giant proteoliposome.
Azioni biochim/fisiol
Sephadex G-10 serves as a gel filtration medium for gel filtration chromatography and protein chromatography. Its versatility is demonstrated by its applications in cancer studies and its ability to determine fluoride levels in diverse samples, including tap water, river water, and green tea samples.
Caratteristiche e vantaggi
- Desalts, removes contaminants, and transfers to a new buffer in one step.
- Suitable for buffer exchange and cleanup of biological samples,
- Effective for peptides, small proteins, and oligosaccharides with a molecular weight larger than 700.
- Efficiently removes salts, dyes, and radioactive labels.
- Lowest exclusion limit of 700 molecular weight in the Sephadex G-range
Note legali
Sephadex is a registered trademark of Cytiva
Prodotto comparabile
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Scegli una delle versioni più recenti:
Possiedi già questo prodotto?
I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
I clienti hanno visto anche
Jinlan Zhang et al.
Journal of food protection, 72(12), 2524-2529 (2009-12-17)
Pentocin 31-1, an anti-Listeria bacteriocin produced by Lactobacillus pentosus 31-1 from the traditional Chinese fermented Xuan-Wei ham, was successfully purified by the pH-mediated cell adsorption-desorption method and then purified by gel chromatography with Sephadex G-10. The purification resulted in a
Kaushal Rege et al.
Nature protocols, 5(3), 408-417 (2010-03-06)
Methods development in chromatography is a time-consuming, trial-and-error process that requires laborious experimentation. We describe a high-throughput screening (HTS) protocol for the rapid identification of chromatographic steps for protein purification from cell-free expression broths. Broths containing the protein are loaded
H Han et al.
Nucleic acids research, 22(14), 2837-2844 (1994-07-25)
Different helical conformations of DNA (D), RNA (R), and DNA.RNA (DR) hybrid double and triple helices have been detected using affinity cleavage analysis. Synthetic methods were developed to attach EDTA.Fe to a single nucleotide on RNA as well as DNA
Kwang Wook Hyun et al.
Peptides, 27(6), 1173-1178 (2005-11-18)
This study describes the extraction and characterization of a platelet aggregation inhibitory peptide from Inonotus obliquus. Ethanol extract from I. obliquus ASI 74006 mycelia showed the highest platelet aggregation inhibitory activity (81.2%). The maximum platelet aggregation inhibitory activity was found
M Muirhead et al.
International journal of cell cloning, 8(6), 392-408 (1990-11-01)
We have cloned a stromal cell from mouse bone marrow selected on the basis of its ability to promote the growth of an Abelson virus-transformed pre-B cell line. These stromal cells have smooth muscle features, and the ultrafiltrate of stromal
Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..
Contatta l'Assistenza Tecnica.