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Key Documents

G25150

Sigma-Aldrich

Sephadex® G-25

Medium

Sinonimo/i:

Sephadex G-25 Medium, Sephadex® G-25 resins, Size exclusion resins

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About This Item

Numero CAS:
Numero MDL:
Codice UNSPSC:
23151817
NACRES:
NA.56

Livello qualitativo

tecniche

buffer exchange: suitable

Gruppo funzionale matrice

phase

Rigonfiamento

1 g swells to 4-6 mL

Dimensioni delle microsfere

50-150 μm

applicazioni

life science and biopharma

Compatibilità

Cytiva

InChI

1S/C6H12O6.C3H8O3/c7-1-2-3(8)4(9)5(10)6(11)12-2;4-1-3(6)2-5/h2-11H,1H2;3-6H,1-2H2/t2-,3-,4-,5-,6-;/m1./s1
BCPVZFFJGCURNR-OCOFDJSDSA-N

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Descrizione generale

Sephadex® G-25 Medium is a size exclusion chromatography or gel filtration resin that is created by crosslinking dextran with epichlorohydrin. The various types of Sephadex can differ in their degree of cross-linking, resulting in variances in their swelling capacity and molecular fractionation range. Sephadex G-25 is one option available among the different G-types of Sephadex. Sephadex G-25 belongs to a range of five G-types, catering to different molecule sizes. Specifically, G-10 is suitable for small molecules, while G-75 is designed to accommodate larger molecules. Sephadex G-25 provides industrial requirements by ensuring a dependable supply and offering extensive technical and regulatory assistance.

Applicazioni

Fractionation Range (MW)
Dextrans: 100 - 5,000
Globular Proteins 1,000 - 5,000

Sephadex® G-25 has been used:
  • in the desalting of protease
  • in the column for the separation of radioiodinated oTP-1 from free iodine during the characterization of radioiodinated oTP-1 and receptor assay validation
Sephadex® G-25 has been used to separate the labelled proteins from unreacted dye N-hydroxysuccinimide (NHS) esters by gel permeation chromatography.

Azioni biochim/fisiol

Sephadex® G-25 is a well-established gel filtration resin, widely used for desalting and buffer exchange in industrial applications. Its hydrophilic matrix minimizes nonspecific adsorption, resulting in high recoveries during these processes for proteins and nucleic acids. Sephadex® G-25 is usually used in affinity chromatography, protein chromatography and gel filtration chromatography.

Caratteristiche e vantaggi

  • Rapid desalting, contaminant removal, and transfer to a new buffer in a single step.
  • High recovery rate with minimal sample dilution.
  • Offered in prepacked HiPrep Desalting and HiTrap desalting columns for convenient and fast desalting.
  • Specifically designed as a BioProcess resin for industrial applications.

Note legali

Sephadex is a registered trademark of Cytiva

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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K M Yocom et al.
Proceedings of the National Academy of Sciences of the United States of America, 79(22), 7052-7055 (1982-11-01)
A stable complex between pentaammineruthenium(III) and histidine-33 in horse heart ferricytochrome c is formed in the reaction between aquopentaammineruthenium(II) and the protein at pH 7. HPLC of the tryptic hydrolysate of the modified protein was employed to identify the pentaammineruthenium
L R Hale et al.
Genetics, 129(1), 103-117 (1991-09-01)
Preliminary studies with restriction fragment length polymorphisms of mitochondrial DNA (mtDNA) in natural populations of Drosophila melanogaster revealed considerable variation in terms of nucleotide sequence and overall size. In this report we present data from more isofemale lines and more
David R Halpin et al.
PLoS biology, 2(7), E173-E173 (2004-06-29)
Recently reported technologies for DNA-directed organic synthesis and for DNA computing rely on routing DNA populations through complex networks. The reduction of these ideas to practice has been limited by a lack of practical experimental tools. Here we describe a
J J Knickerbocker et al.
Biology of reproduction, 40(2), 361-369 (1989-02-01)
Scatchard analysis was used to determine the distribution, number, and affinity of unoccupied receptors for ovine trophoblast protein-1 (oTP-1) in endometrium of sheep throughout the estrous cycle and early pregnancy. In Experiment I, oTP-1 receptor characteristics were determined in membrane
Characterization of endometrial receptors for ovine trophoblast protein-1 during the estrous cycle and early pregnancy in sheep
Knickerbocker JJ and Niswender GD
Biology of Reproduction, 40(2), 361-369 (1989)

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