XNACRL

Extract-N-Amp^™ Cellular RNA Lysis Buffer

Optimized for rapid cell lysis and RNA stabilization

• Sinonimo/i: RNA extraction buffer, RNA isolation buffer, RNA lysis buffer
• Codice UNSPSC: 41106306

Proprietà

• Livello qualitativo: 200
• Forma fisica: liquid
• Condizioni di stoccaggio: OK to freeze (-10 to-25°C)
• Compatibilità: suitable for PCR (suitable for RT-qPCR)
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

The Extract-N-AmpTM Cellular RNA Lysis buffer (XNACRL) is a single working solution that allows for rapid, room-temperature extraction and stabilization of RNA from a range of cells without heat, agitation or clean up. This is a simple and robust method for preparing cell lysate and stabilizing the extracted RNA for gene expression analysis directly from the cell lysate without the need for purification step prior to analysis.

Applicazioni

• The Extract-N-AmpTM Cellular RNA Lysis buffer is suitable for direct use with
• qRT-PCR
• RT-LAMP
• NGS library preparation

Caratteristiche e vantaggi

• From cell lysis to qRT-PCR in 15 minutes
• High Throughput and automation-friendly
• No RNA purification or clean up necessary
• No columns, heat, or centrifugation necessary
• Compatible with both adherent and non-adherent mammalian cell types
• Sensitivity range: 10-100k cells •May be paired with DNase I to ensure removal of DNA for more sensitive processes

Stato fisico

Liquid

Stoccaggio e stabilità

Freezer (-10 to-25°C)

Note legali

Extract-N-Amp is a trademark of Sigma-Aldrich Co. LLC

Solo come componenti del kit

• DNA-free JumpStart Taq DNA Polymerase 250U, 1.5KU
• DNA-free JumpStart Taq DNA Polymerase