SRP0364
mTOR/Raptor/MLST8 human
recombinant, expressed in baculovirus infected Sf9 cells, ≥50% (SDS-PAGE)
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About This Item
Codice UNSPSC:
12352200
NACRES:
NA.32
Prodotti consigliati
Origine biologica
human
Ricombinante
expressed in baculovirus infected Sf9 cells
Saggio
≥50% (SDS-PAGE)
Stato
aqueous solution
PM
137 kDa (mTOR)
150 kDa (Raptor)
37 kDa (MLST8)
Confezionamento
pkg of 10 μg
Produttore/marchio commerciale
Sigma-Aldrich
tecniche
cell based assay: suitable
Solubilità
water: soluble
N° accesso NCBI
applicazioni
life science and biopharma
Condizioni di spedizione
dry ice
Temperatura di conservazione
−70°C
Informazioni sul gene
human ... MLST8(64223) , MTOR(2475) , RPTOR(57521)
Descrizione generale
This product (recombinant mTOR/Raptor/MLST8) is a complex of human mechanistic target of rapamycin (mTOR), also known as FRAP1 (GenBank Accession No. NM_004958), amino acids 1362-end with N-terminal FLAG-tag, MW = 137 kDa; human mTOR associated protein, LST8 homolog (MLST8) (GenBank Accession No. NM_022372) amino acids 2-326 (end) with N-terminal His-tag, MW = 37 kDa and human regulatory associated protein of MTOR complex 1 (Raptor) (GenBank Accession No. NM_020761) amino acids 2-1335 (end) with N-terminal His-tag, MW=150 kDa, co-expressed in a Baculovirus-infected Sf9 cell expression system.
Applicazioni
Recombinant mTOR/Raptor/MLST8 has been used in in vitro caspase reactions.
Azioni biochim/fisiol
Mechanistic target of rapamycin (mTOR) modulates cell growth and division by integrating nutrient and mitogen signals. It also modulates the functions of antigen-presenting cells, like dendritic cells and activates T-cells. mTOR is also involved in innate and adaptive immunity. mTOR associated protein, LST8 homolog (MLST8) is a subunit of both the mTOR complex 1 and 2 (mTORC1 and 2). It is crucial for the activation of the mTOR kinase and is a regulator of mTOR pathways. Regulatory-associated protein of mTOR complex 1 (RAPTOR) has an important role in modulating cell growth and energy homeostasis as a scaffolding protein. It takes part in recruiting substrates of the mechanistic target of rapamycin (mTOR) to the rapamycin-sensitive mTOR complex 1 (mTORC1). RAPTOR also associates with proteins involved in the pathway and aids in phosphorylating them.
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
Immunoregulatory functions of mTOR inhibition.
Thomson AW
Nature Reviews: Immunology, 9(5), 324-337 (2009)
Belen Rubio-Viqueira et al.
Advances in experimental medicine and biology, 587, 309-327 (2006-12-14)
The mammalian target of rapamycin (mTOR) is involved in the control of cellular growth and proliferation. Abnormal activation of signaling pathways both proximal and distal to this kinase occurs frequently in human cancer suggesting that mTOR is an attractive target
mTOR controls cell cycle progression through its cell growth effectors S6K1 and 4E-BP1/eukaryotic translation initiation factor 4E.
Fingar DC
Molecular and Cellular Biology, 24(1), 200-216 (2004)
mLST8 Promotes mTOR-Mediated Tumor Progression.
Kakumoto K
PLoS ONE, 10(4), e0119015-e0119015 (2015)
Lourdes Toral-Barza et al.
Biochemical and biophysical research communications, 332(1), 304-310 (2005-05-18)
The mammalian target of rapamycin (mTOR/TOR) is implicated in cancer and other human disorders and thus an important target for therapeutic intervention. To study human TOR in vitro, we have produced in large scale both the full-length TOR (289 kDa)
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