P9290
Proteinase K–Agarose from Tritirachium album
lyophilized powder
Sinonimo/i:
Proteinase K-Agarose
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About This Item
Prodotti consigliati
Origine biologica
microbial (T.album
T. ALBUM)
Livello qualitativo
Forma fisica
lyophilized powder
Grado di funzionalizzazione
30-140 units per g solid
tecniche
DNA extraction: suitable
RNA extraction: suitable
protein extraction: suitable
Rigonfiamento
1 g swells to ~5 mL
Temperatura di conservazione
−20°C
Applicazioni
Proteinase K-agarose is used in protein and nucleic acid isolation, modifying enzymes and immobilized enzymes. Proteinase K-agarose has been used to study Klebsiella pneumonia as well as Echinococcus multilocularis infection in domesticated dogs.
Azioni biochim/fisiol
The application of proteinase K has been extended by immobilizing it on an insoluble matrix such as agarose. The agarose is quite inert in most systems and allows high activities per unit weight. This reactive resin allows the enzyme reaction to be quickly catalyzed by momentary contact with the substrate in a suitable medium. Reactions can be carried out in a batch slurry or even through a small column. In either case, the insoluble enzyme does not contaminate the reaction mix, which can be readily filtered out of the batch slurry or remain behind in the column.
Caratteristiche e vantaggi
• Recylable Proteinase K on matrix support.
• Spin column or batch processing possible.
• Spin column or batch processing possible.
Definizione di unità
One unit will hydrolyze urea-denatured hemoglobin to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent).
Stato fisico
Lyophilized powder stabilized with lactose
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
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Hirokazu Kouguchi et al.
Experimental parasitology, 128(1), 50-56 (2011-02-09)
We show that a conventionally purified glycoprotein component of Echinococcus multilocularis protoscolex, designated as Emgp-89, may be useful as a serodiagnostic antigen for detecting E. multilocularis infection in dogs domesticated in endemic areas. Emgp-89 was obtained from the parasite material
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Jenab, A., et al.
Arch. Immunol. Ther. Exp., 58, 227-234 (2010)
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This report describes a method for preparing both DNA and RNA simultaneously from as few as 5 X 10(5) lymphoid cells. The method is suitable for cultured cells or any tissue from which a cell suspension can be prepared and
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