P5459
Proteasi
glycerol solution (50%)
Sinonimo/i:
Proteinasi, Subtilisina A
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About This Item
Numero CAS:
Numero MDL:
Codice UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Prodotti consigliati
Origine biologica
Bacillus licheniformis
Livello qualitativo
Stato
glycerol solution (50%)
Attività specifica
≥7 units/mg protein (biuret)
Concentrazione
≥50 mg/mL
Solubilità
0.85% sodium chloride: soluble, clear (Diluted)
H2O: miscible
Temperatura di conservazione
2-8°C
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Descrizione generale
The enzyme hydrolyzes gelatin optimally at 50 °C over the range of pH 8-10. It is stable between pH 6-10 at 50 °C. Commercially, it is used in laundry detergent formulations and also for the recovery of silver from photographic emulsions.
Applicazioni
Protease from Bacillus licheniformis has been used to digest the neurolemma in the antennal lobe of the moth; Heliothis virescens.
Protease is an enzyme used to break down proteins by hydrolyzing peptide bonds. Protease is used to degrade proteins, to study protease inhibitors and to study thermal inactivation kinetics. Protease is used in nucleic acid isolation procedures in incubations. Protease from Bacillus licheniformis, product P5459, has been used to hydrolyze muscle during the isolation of mitochondria from skeletal muscle.
Azioni biochim/fisiol
Protease catabolizes proteins by hydrolysis of peptide bonds. Proteases are inactivated by serine active-site inhibitors, such as phenylmethylsulfonyl fluoride (PMSF) and diisopropylfluorophosphate.
Confezionamento
Package size based on protein content.
Definizione di unità
One unit will hydrolyze casein to produce color equivalent to 1.0 μmole (181 μg) of tyrosine per min at pH 7.5 at 37 °C (color by Folin-Ciocalteu reagent), unless otherwise indicated.
Avvertenze
Danger
Indicazioni di pericolo
Consigli di prudenza
Classi di pericolo
Aquatic Chronic 3 - Eye Dam. 1 - Resp. Sens. 1
Codice della classe di stoccaggio
10 - Combustible liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Dispositivi di protezione individuale
dust mask type N95 (US), Eyeshields, Faceshields, Gloves
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Protocolli
To standardize a procedure for the enzymatic assay of Protease using Casein as a substrate.
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