L8146
Lectin from Ulex europaeus (gorse, furze)
peroxidase conjugate, lyophilized powder
Sinonimo/i:
Ulex europaeus agglutinin, UEA-I
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Origine biologica
Ulex europaeus
Livello qualitativo
Coniugato
peroxidase conjugate
Saggio
70.00-90.00% (Lowry)
Forma fisica
lyophilized powder
Potenza
<40 μg/mL agglutination activity
peroxidase activity
≥15 units/mg protein
Composizione
Protein, ~80% Lowry
tecniche
western blot: suitable
Temperatura di conservazione
2-8°C
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Applicazioni
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Azioni biochim/fisiol
Ulex europaeus agglutinin has anti-H blood group specificity. There are two types of lectin: UEA I has an affinity for L-fucose and UEA II has an affinity for N,N′-diacetylchitobiose. The mol. wt. of UEA I was initially found to be 170,000 daltons. Later reports indicate that UEA I may form aggregates at neutral and basic pH and that the correct mol. wt. is 68,000 daltons.
Confezionamento
Package size based on protein content.
Linkage
Conjugates are prepared from affinity purified lectin.
Definizione di unità
One unit will form 1.0 mg purpurogallin in 20 sec from pyrogallol at pH 6.0 at 20 °C.
Stato fisico
Contains sodium citrate buffer salts
Nota sulla preparazione
Prepared from peroxidase, Type VI (P8375), using the two-step glutaraldehyde method of Avrameas and Ternyck.
Repurified by affinity chromatography after conjugation.
Repurified by affinity chromatography after conjugation.
Risultati analitici
Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group O erythrocytes after 1 hour incubation at 25 °C.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
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