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L9640

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

lyophilized powder

Sinonimo/i:

WGA, Wheat germ agglutinin

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Forma fisica

lyophilized powder

Livello qualitativo

300

Potenza

<20 μg/mL agglutination activity

Composizione

Protein, 100% biuret

Impurezze

salt, essentially free

Temperatura di conservazione

2-8°C

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Azioni biochim/fisiol

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Nota sulla preparazione

Conjugates are prepared from affinity purified lectin.

Risultati analitici

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg/ml solution. This activity is the lowest concentration to agglutinate a 2% suspension of human erythrocytes after 1 hour incubation at 25 °C.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Endoscopic surveillance for Barrett's esophagus (BE) is limited by long procedure times and sampling error. Near-infrared (NIR) fluorescence imaging minimizes tissue autofluorescence and optical scattering. We assessed the feasibility of a topically applied NIR dye-labeled lectin for the detection of
Julie Beneteau et al.
Plant physiology, 153(3), 1345-1361 (2010-05-06)
Phloem Protein2 (PP2) is a component of the phloem protein bodies found in sieve elements. We describe here the lectin properties of the Arabidopsis (Arabidopsis thaliana) PP2-A1. Using a recombinant protein produced in Escherichia coli, we demonstrated binding to N-acetylglucosamine
Jiamao Lin et al.
European journal of pharmacology, 859, 172490-172490 (2019-06-24)
Doxorubicin (DOX) is a classic anti-tumor chemotherapeutic used to treat a wide range of tumors. One major downfall of DOX treatment is it can induce fatal cardiotoxicity. Astragaloside IV (AS-IV) is one of the primary active ingredients that can be
Isolation and purification of serum lipoprotein (a) using lectin affinity.
L J Seman et al.
Methods in molecular biology (Clifton, N.J.), 110, 131-136 (1999-01-26)
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Biomedical optics express, 3(4), 753-763 (2012-05-11)
Two microparticles were biochemically attached to a red blood cell at diametrically opposite parts and held by optical traps allowing to impose deformations. The cell deformation was monitored from the microscopy images. Raman spectra of the cell under tunable deformations
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