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Key Documents

L4895

Sigma-Aldrich

Lectin from Triticum vulgaris (wheat)

FITC conjugate, lyophilized powder

Sinonimo/i:

WGA, Wheat germ agglutinin

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About This Item

Codice UNSPSC:
12352202
NACRES:
NA.32

Coniugato

FITC conjugate

Livello qualitativo

Forma fisica

lyophilized powder

Potenza

<50 μg/mL agglutination activity

Composizione

Protein, ~50% Lowry

Grado di funzionalizzazione

~2 mol FITC per mol protein

Temperatura di conservazione

2-8°C

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Descrizione generale

Lectins are carbohydrate-binding proteins. They are omnipresent and usually found in fungi, plants and animals. The secondary structure of this protein is rich in β-strands and possesses carbohydrate binding sites on the surface.

Applicazioni

For the fluorescent detection of glycoproteins containing β(1→4)-N-acetyl-D-glucosamine
Lectin from Triticum vulgaris (wheat) has been used:
  • to treat transfected cells for immunofluorescence
  • to stereotaxically inject into mice for neuronal tracing
  • in immunohistochemistry for the analysis of myocytes

Azioni biochim/fisiol

Lectin plays a key role in glycoconjugate characterizing, imaging and targeting. Its use in a microarray assay, enable efficient glycome profiling. In plants and fungi, lectin defends against pathogens/feeders. Lectin also plays a major role in host recognition and tissue adhesion.
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Confezionamento

Package size based on protein content.

Stato fisico

Contains phosphate buffer salts

Nota sulla preparazione

Conjugates are prepared from affinity purified lectin.

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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