L3892
Lectin from Triticum vulgaris (wheat)
peroxidase conjugate, lyophilized powder
Sinonimo/i:
WGA, Wheat germ agglutinin
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About This Item
Codice UNSPSC:
12352202
NACRES:
NA.32
Prodotti consigliati
Origine biologica
Triticum vulgaris
Livello qualitativo
Coniugato
peroxidase conjugate
Saggio
≥85% protein basis (Warburg-Christian)
Forma fisica
lyophilized powder
Potenza
<40 μg/mL agglutination activity
peroxidase activity
50-200 units/mg protein
Composizione
Protein, ~90% modified Warburg-Christian
Temperatura di conservazione
−20°C
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Applicazioni
Detects glycoproteins containing β(1→4)-N-acetyl-D-glucosamine when used with appropriate peroxidase substrate
General Western Blot Protocol:
- Glycoprotein sample size: 500ng
- Lectin Concentration: 0.1ug/ml
- Load samples at 500 ng of glycoprotein per lane
- Run 4-20% Bis-Tris SDS page gel
- Transfer gel to a PVDF membrane
- Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
- Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
- Wash membrane 5 x 5 minutes with 25ml RIPA buffer
- Detect using chemiluminescent substrate (CPS1-120)
Azioni biochim/fisiol
WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.
Confezionamento
Package size based on protein content.
Definizione di unità
One unit will form 1.0 mg of purpurogallin from pyrogallol in 20 sec at pH 6.0 at 20 °C.
Stato fisico
Contains citrate buffer
Nota sulla preparazione
Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.
Codice della classe di stoccaggio
11 - Combustible Solids
Classe di pericolosità dell'acqua (WGK)
WGK 3
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Dispositivi di protezione individuale
Eyeshields, Gloves, type N95 (US)
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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