L1882

Lectin from Triticum vulgaris (wheat)

agarose conjugate, saline suspension

• Sinonimo/i: WGA, Wheat germ agglutinin
• Codice UNSPSC: 12352202
• NACRES: NA.32

Proprietà

• Coniugato: agarose conjugate
• Livello qualitativo: 200
• Stato: saline suspension
• Grado di funzionalizzazione: 5-10 mg per mL
• Matrice: cross-linked 4% beaded agarose
• Attivazione matrice: cyanogen bromide
• Capacità: ≥0.1 mg/mL binding capacity (ovomucoid)
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Lectins are carbohydrate-binding proteins, found in fungi, plants and animals. The secondary structure of a lectin is rich in β-strands and possesses a carbohydrate binding sites on the surface.

Applicazioni

Used for the purification of glycoproteins containing (D-N-acetylglucosamine)₂ or N-acetylneuraminic acid.

Azioni biochim/fisiol

Lectins are known to be useful in glycoconjugate characterizing, imaging and targeting. They specifically interact with oligosaccharides, glycoproteins and glycolipids. Hence, lectins are used in microarray assays, enabling efficient glycome profiling. In plants and fungi, lectins defend against pathogens or feeders. They are also involved in host recognition and tissue adhesion.

WGA is not blood group specific but has an affinity for N-acetyl-β-D-glucosaminyl residues and N-acetyl-β-D-glucosamine oligomers. WGA contains no protein-bound carbohydrate.

Stato fisico

Suspension in 1.0 M NaCl containing 15 mM sodium azide

Nota sulla preparazione

Conjugates are prepared from affinity purified lectin.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Dispositivi di protezione individuale: Eyeshields, Gloves