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GE17-0444-01

Lentil Lectin-Sepharose 4B 25 mL

Cytiva 17-0444-01

Sinonimo/i:

Sepharose 4B

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

ligand

lentil lectin

Confezionamento

pkg of 25 mL

Produttore/marchio commerciale

Cytiva 17-0444-01

Condizioni di stoccaggio

(20% Ehtanol, PBS pH 7)

Matrice

4% agarose

Dimensione particelle

45-165 μm

Diametro medio

90 μm

cleaning in place

3-12

Intervallo di esercizio

3-10

Temperatura di conservazione

2-8°C

Descrizione generale

Lentil Lectin Sepharose media is used for purification of a number of glycoproteins and other carbohydrate containing molecules. The lentil lectin ligand has more equal affinity for α-D-mannose and α-D-glucose compared to Con A.

Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars. Lentil Lectin Sepharose 4B is a generally applicable group specific adsorbent and is routinely used in the preparation and purification of a number of glycoproteins and carbohydrate containing molecules.

Caratteristiche e vantaggi

  • Binds molecules that contain α-D-mannose, α-D-glucose, and sterically related residues
  • Compared to Con A, also for glycoprotein purification, Lentil Lectin distinguishes less sharply between glucosyl and mannosyl
  • Retains binding in 1% deoxycholate and is especially valuable for purification of detergent-solubilized membrane proteins
  • Lentil lectin is a metalloprotein, containing Ca2+ and Mn2+, which binds reversibly to polysaccharides and glycoconjugates containing glucose or mannose type sugars

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

Sepharose is a trademark of Cytiva

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Warning

Indicazioni di pericolo

Codice della classe di stoccaggio

3 - Flammable liquids


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Articoli

This page shows how to solve practical problems that may occur when running an affinity chromatography column.

Protocolli

This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from GE Healthcare.

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

This page shows how to perform column packing and preparation for affinity chromatography when using Tricorn™ or XK columns available from GE Healthcare.

This page shows how to prepare samples for purification with affinity chromatography.

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