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P8651

Sigma-Aldrich

Protein A–Peroxidase from Staphylococcus aureus/horseradish

lyophilized powder

Sinonimo/i:

Peroxidase–Protein A

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About This Item

Numero MDL:
MFCD00132102
Codice UNSPSC:
12352203
NACRES:
NA.46

Coniugato

peroxidase conjugate

Livello qualitativo

100
200

Forma fisica

lyophilized powder

tecniche

direct ELISA: 1:80,000 using human IgG

Temperatura di conservazione

−20°C

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Descrizione generale

Protein A is a cell wall constituent of Staphylococcus aureus, with a molecular weight of 56kDa. It possesses five binding domains which interact with heavy chains of immunoglobulins at the Fc or Fab fragment. In response to protein A, the complement system, interferon production, hypersensitivity reactions and cell-mediated cytotoxicity get activated. Horseradish peroxidase is a heme-containing enzyme. In the presence of hydrogen peroxide, the enzyme catalyzes the oxidation of luminol.

Specificità

Binds IgG only from most mammals, except rat, goat, sheep.

Applicazioni

Protein A–Peroxidase from Staphylococcus aureus/horseradish has been used in enzyme-linked immunosorbent assay (ELISA).

Confezionamento

Package size based on protein content.

Stato fisico

Lyophilized powder containing sodium citrate buffer salt. Protein determined by A205.

Nota sulla preparazione

Protein A, extracellular, coupled to Peroxidase, Type VI, by a modification of the procedure of O′Sullivan, M.J., et al., FEBS Lett., 95, 311 (1978), which favors low molecular weight conjugates.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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Staphylococcal protein A consists of five IgG-binding domains
Tomas MOKS
European Journal of Biochemistry (1986)
Katharina Mayer et al.
International journal of molecular sciences, 22(16) (2021-08-28)
Within-host adaptation is a typical feature of chronic, persistent Staphylococcus aureus infections. Research projects addressing adaptive changes due to bacterial in-host evolution increase our understanding of the pathogen's strategies to survive and persist for a long time in various hosts
D.P. Nikoleis
Biosensors for Direct Monitoring of Environmental Pollutants in Field (2013)
P Eliades et al.
Journal of immunological methods, 218(1-2), 123-132 (1998-11-18)
We have developed a dot immunobinding assay (CM) for the serodiagnosis of the human hydatidosis. The assay employs Samaron blue colloidal dye particles conjugated to hydatid antigen (HA) or protein A, both of which serve as visualizing agents. The test
Greg T
Bioconjugate Techniques (2013)
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Protocolli

Enzymatic Assay of Peroxidase (EC 1.11.1.7) 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a Substrate

To standardize a procedure for the assay of Peroxidase using 2,2'-Azino-bis(3-Ethylbenzthiazoline-6-Sulfonic Acid) as a substrate.

Enzymatic Assay of Peroxidase (EC 1.11.1.7)

This procedure is for the determination of Peroxidase enzymatic activity using Pyrogallol as the substrate.

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