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Sigma-Aldrich

Peroxidase from horseradish

lyophilized, powder, ~150 U/mg

Sinonimo/i:

(2R,3S,6S,7R,8R)-3-(3-formamido-2-hydroxybenzamido)-8-hexyl-2,6-dimethyl-4,9-dioxo-1,5-dioxonan-7-yl 3-methylbutanoate, Antimycin A, Horseradish peroxidase

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

horseradish

Forma fisica

powder

Qualità

lyophilized

Attività specifica

~150 U/mg

PM

Mr ~40000

Solubilità

50 mM potassium phosphate buffer, pH 6.0: 5 mg/mL (0.1% BSA)

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

InChI

1S/H2O3/c1-3-2/h1-2H
JSPLKZUTYZBBKA-UHFFFAOYSA-N

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Descrizione generale

Horseradish peroxidase (HRP) is a vital and a widely studied heme-containing enzyme, isolated from a hardy perennial herb, horse radish. It catalyzes the oxidation of various organic and inorganic compounds by using hydrogen peroxide as substrates. HRP is involved in indole-3-acetic acid metabolism, cross-linking of biological polymers and lignification. This enzyme in combination with indole-3-acetic acid or its derivatives acts as a potential therapeutic for targeted cancer therapies. Commercially HRP is used as a constituent of clinical diagnostic kits and is used in immunoassays.

Applicazioni

Peroxidase from horseradish has been used:
  • for isolation of N-Linked glycans
  • as a constituent of D-amino acid oxidase (DAAO) buffer
  • to decompose hydrogen peroxide (H2O2) into water to demonstrate the performance of the portable oxidative stress sensor (POSS)

Horseradish peroxidase is used widely within biochemistry as a detection reagent in a variety of applications including ELISA, immunoblotting, and immunohistochemistry.

Definizione di unità

1 U corresponds to the amount of enzyme which oxidizes 1 μmol ABTS (Cat. No. 11557) per minute at pH 6.0 and 25°C

Altre note

Use of chemically modified peroxidase in benzene; Cross-linking of proteins; Enzyme catalyzed polymerization and precipitation of aromatic compounds from wastewater

Sostituito da

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

13 - Non Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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