10814407001

Peroxidase (POD)

EIA grade, from horseradish

• Sinonimo/i: POD
• Classificazione EC (Enzyme Commission): 1.11.1.7 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204

Proprietà

• Origine biologica: horseradish
• Livello qualitativo: 100
• Saggio: 90% (HPLC)
• Forma fisica: lyophilized (salt-free)
• Attività specifica: ~225 U/mg lyophilizate, 25 °C (Guaiacol, H2O2)
• Confezionamento: pkg of 100 mg
• Produttore/marchio commerciale: Roche
• Concentrazione: 10 g/L
• tecniche: activity assay: suitable
• Colore: red to brown
• pH ottimale: 6.0-6.5
• Solubilità: water: soluble at 68 °F
• Compatibilità: suitable for molecular biology
• N° accesso UniProt: P00433
• applicazioni: detection; life science and biopharma
• Attività estranea: ATPase <0.00100%, none detected; AcP <0.00100%; Catalase <0.700%
• Condizioni di spedizione: dry ice
• Temperatura di conservazione: −20°C (−15°C to −25°C)

Descrizione

Descrizione generale

Donor: hydrogen-peroxide oxidoreductase
Peroxidase can be coupled to other proteins via its amino groups, as well as its carbohydrate moiety. The reconstituted solution can be used directly for conjugation without prior dialysis.

Applicazioni

Peroxidase (POD) has been used in the spermine oxidase enzyme activity assay.

Sequenza

Blocking substances
Preparations does not contain substances that block the amino groups, but in contrast to other enzymes POD has only 2 to 3 free amino groups which are not accessible; even by extended dialysis of POD, the number of NH₂-groups/mol does not change.

POD
POD contains 1 molecule of protoheme IX as prosthetic group and 2 molecules of Ca²⁺.
POD exists in > 10 isoenzymic forms (some sources estimate approx. 40 isoenzymes).

Carbohydrate Content
12 to 14.5 mg/100 mg Iyophilizate

Free Amino Groups
Free amino groups: 2 to 3 mol/mol enzyme

Nota sulla preparazione

Activator: Ammonia, pyridine, imidazole, all at pH >7; detergents
Working concentration: A suggested working ratio, for immediate use in coupling procedures, is 2 mg POD to 4 mg lgG (molar ratio = 2:1, POD:IgG).
Specific activity: approximately 225U/mg lyophilizate (+25°C, guaiacol, H₂O₂) approximately 1,000 U/mg lyophilizate (+25°C, ABTS, H₂O₂, pH 5.0) approximately 3,500 U/mg lyophilizate (+25°C, tetramethylbenzidine, H₂O₂)
Amino groups: 2 - 3moles/mole enzyme

Carbohydrates: 12.0 - 14.5% (w/w)
Isoenzymes: ≥90% isoenzyme C (HPLC)
Purity: single peak (HPLC)
Purity number: 3.0 - 3.5 (A₄₀₃/A₂₇₅)

Stoccaggio e stabilità

Keep container tightly closed in a dry and well-ventilated place.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H317,H334
• Consigli di prudenza: P261 - P280 - P284 - P304 + P340 - P333 + P313 - P342 + P311
• Classi di pericolo: Resp. Sens. 1 - Skin Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable