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H4272

Sigma-Aldrich

Ialuronidasi

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

Sinonimo/i:

Ialuronato 4-glicanoidrolasi, Ialuronoglucosaminidasi

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About This Item

Numero CAS:
37326-33-3
Classificazione EC (Enzyme Commission):
3.2.1.35 (BRENDA, IUBMB)
Numero CE:
253-464-3
Numero MDL:
MFCD00131351
Codice UNSPSC:
12352204
NACRES:
NA.75

Sterilità

aseptically processed

Livello qualitativo

200

Tipo

Type IV-S

Stato

powder

Attività specifica

750-3000 units/mg solid

PM

~55 kDa (four subunits of 14 kDa each)

Composizione

Protein, 80-105% biuret

tecniche

cell culture | embryo: suitable

Temperatura di conservazione

−20°C

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Applicazioni

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

Azioni biochim/fisiol

La ialuronidasi degrada lo ialuronano ed è stato scoperto che è regolata in maniera inappropriata durante la progressione del cancro.
Questi enzimi scindono casualmente i legami glicosidici della β-N-acetilesosammina-[1→4] nell′acido ialuronico, nella condroitina e nei condroitina solfati.

Definizione di unità

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Nota sulla preparazione

Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.

Raccomandato

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Health hazard
GHS08

Avvertenze

Danger

Indicazioni di pericolo

H334

Consigli di prudenza

P261 - P284 - P501

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

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Certificati d'analisi (COA)

Lot/Batch Number
SLCJ3456
SLCJ0558
SLCD4050
SLCC6227
SLBX1080

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M16 Medium With sodium bicarbonate and lactic acid, without penicillin and streptomycin, liquid, sterile-filtered

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M16 Medium

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Hyaluronidase, Streptomyces hyaluronlyticus nov. sp.

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M2 medium EmbryoMax M2 Medium (1X), Liquid, with Phenol Red & Hyaluronidase

Sigma-Aldrich

MR-051

M2 medium

Olio minerale light oil, suitable for mouse embryo cell culture, BioXtra

Sigma-Aldrich

M5310

Olio minerale

Collagenasi suitable for release of physiologically active rat epididymal adipocytes, Type II, 0.5-5.0 FALGPA units/mg solid, ≥125 CDU/mg solid

Sigma-Aldrich

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Collagenasi

Olio minerale light oil, (neat)

Sigma-Aldrich

M8410

Olio minerale

Liesl Jacobs et al.
Molecular therapy : the journal of the American Society of Gene Therapy, 28(4), 1068-1077 (2020-02-27)
Checkpoint-inhibiting antibodies elicit impressive clinical responses, but still face several issues. The current study evaluated whether DNA-based delivery can broaden the application of checkpoint inhibitors, specifically by pursuing cost-efficient in vivo production, facilitating combination therapies, and exploring administration routes that lower
Yu Shi et al.
Nature communications, 8(1), 2043-2043 (2017-12-13)
Bone formation in mammals requires continuous production of osteoblasts throughout life. A common molecular marker for all osteogenic mesenchymal progenitors has not been identified. Here, by lineage-tracing experiments in fetal or postnatal mice, we discover that Gli1
Wan Kin Au Yeung et al.
Cell reports, 27(1), 282-293 (2019-04-04)
Mammalian histone methyltransferase G9a (also called EHMT2) deposits H3K9me2 on chromatin and is essential for postimplantation development. However, its role in oogenesis and preimplantation development remains poorly understood. We show that H3K9me2-enriched chromatin domains in mouse oocytes are generally depleted
Marco Ragusa et al.
RNA biology, 16(9), 1237-1248 (2019-05-29)
Circular RNAs (circRNAs) have a critical role in the control of gene expression. Their function in spermatozoa (SPZ) is unknown to date. Twenty-eight genes, involved in SPZ/testicular and epididymal physiology, were given in circBase database to find which of them
P Lorenzo Bozzelli et al.
Experimental neurology, 323, 113077-113077 (2019-11-05)
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