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H4272

Sigma-Aldrich

Ialuronidasi

Type IV-S, powder, suitable for mouse embryo cell culture, 750-3000 units/mg solid

Sinonimo/i:

Ialuronato 4-glicanoidrolasi, Ialuronoglucosaminidasi

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About This Item

Numero CAS:
Classificazione EC (Enzyme Commission):
Numero CE:
Numero MDL:
Codice UNSPSC:
12352204
NACRES:
NA.75

Sterilità

aseptically processed

Livello qualitativo

Tipo

Type IV-S

Stato

powder

Attività specifica

750-3000 units/mg solid

PM

~55 kDa (four subunits of 14 kDa each)

Composizione

Protein, 80-105% biuret

tecniche

cell culture | embryo: suitable

Temperatura di conservazione

−20°C

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Applicazioni

Hyaluronidase is an enzyme derived from bovine testes. It is used to remove the cumulus mass in the isolation of mouse embryos or from newly ovulated eggs, when preparing the eggs for in vitro culture.

Gonadotropins are used to superovulate 20 female mice. The mice are then mated and preimplantation stage embryos are retrieved and counted. The embryos are treated with hyaluronidase until the cumulus mass is dissociated from the embryos. The embryos are cultured, viewed daily and progress of the embryos is recorded.

Azioni biochim/fisiol

La ialuronidasi degrada lo ialuronano ed è stato scoperto che è regolata in maniera inappropriata durante la progressione del cancro.
Questi enzimi scindono casualmente i legami glicosidici della β-N-acetilesosammina-[1→4] nell′acido ialuronico, nella condroitina e nei condroitina solfati.

Definizione di unità

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Nota sulla preparazione

Reconstitute 1 vial of hyaluronidase with 3 ml of M2 Medium (Product Nos. M 7167 or M 5910) or deionized water to obtain a 10 mg/ml stock solution. Dilute stock solution with M2 Medium to obtain a final working concentration of approximately 300 μg/ml.

Pittogrammi

Health hazard

Avvertenze

Danger

Indicazioni di pericolo

Consigli di prudenza

Classi di pericolo

Resp. Sens. 1

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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Articoli

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Protocolli

This procedure may be used for Hyaluronidase products.

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