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H1136

Sigma-Aldrich

Hyaluronidase from Streptomyces hyalurolyticus

Sinonimo/i:

Hyaluronate Lyase from Streptomyces hyalurolyticus

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About This Item

Numero CAS:
37259-53-3
Classificazione EC (Enzyme Commission):
4.2.2.1 (BRENDA, IUBMB)
Numero CE:
253-430-8
Numero MDL:
MFCD00131347
Codice UNSPSC:
12352204
NACRES:
NA.54

Origine biologica

Streptomyces sp. (S. hyalurolyticus)

Livello qualitativo

200

Coniugato

conjugate (Glucosaminoglycan)

Forma fisica

film (Clear)

Confezionamento

ampule of 300 ×  units

Temperatura di conservazione

−20°C

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Categorie correlate

Descrizione generale

Hyaluronidase is widely present in nature. In humans, it is present in a number of organs and body fluids. Hyaluronidase is found in the secretions of virus, bacteriophage, fungi and bacteria. It is also present in nematode and leeches secretions. Snakes and scorpions produce hyaluronidase in their venom. Hyaluronidase is also secreted by bees, spiders, wasps, hornets, caterpillars, fishes and lizards.

Applicazioni

Hyaluronidase from Streptomyces hyalurolyticus has been used:
  • in the preparation of DMEM/F-12 (Dulbecco′s modified eagle medium/nutrient mixture F-12) media for the isolation and purification of single cells from dissociated tumour tissue
  • as a component of digestion solution for the derivation of germline stem cells from testicular tissue

Azioni biochim/fisiol

Hyaluronate lyase cleaves hyaluronic acid at the β-D-GlcNAc-(1→4)-β-D-GlcA bond, yielding 3-(4-deoxy-β-D-gluc-4-enuronosyl)-N-acetyl-D-glucosamine tetra- and hexasaccharides. Unlike other hyaluronidases, this enzyme is specific for hyaluronic acid and is inactive with chondroitin and chondroitin sulfate.
Hyaluronidase is an endoglycosidase and performs its function along with exoglycosidases, like β-glucuronidase and β-N-acetyl glucosaminidase. Hyaluronidase regulates the hyaluronic acid turnover.

Definizione di unità

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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CD44v8-10 is a cancer-specific marker for gastric cancer stem cells
Lau WM, et al.
Cancer Research, canres-ca2309 (2014)
Conversion of adult mouse unipotent germline stem cells into pluripotent stem cells
Ko K, et al.
Nature Protocols, 5(5), 921-921 (2010)
Caitlin E Henderson-Toth et al.
Developmental biology, 369(2), 330-339 (2012-07-24)
The glycocalyx, and the thicker endothelial surface layer (ESL), are necessary both for endothelial barrier function and for sensing mechanical forces in the adult. The goal of this study is to use a combination of imaging techniques to establish when
Wen Min Lau et al.
Cancer research, 74(9), 2630-2641 (2014-03-13)
The surface marker CD44 has been identified as one of several markers associated with cancer stem cells (CSC) in solid tumors, but its ubiquitous expression in many cell types, including hematopoietic cells, has hindered its use in targeting CSCs. In
Swathi Balaji et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 31(3), 868-881 (2016-12-03)
The cytokine IL-10 has potent antifibrotic effects in models of adult fibrosis, but the mechanisms of action are unclear. Here, we report a novel finding that IL-10 triggers a signal transducer and activator of transcription 3 (STAT3)-dependent signaling pathway that
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Articoli

Carbohydrate Analysis III

A key resource feature at our Enzyme Explorer section of biochemicals is "Enzymes for Carbohydrate Analysis and Digestion." Offering kits, reagents, analysis, lists of enzymes related to PTM and carbohydrate metabolism.

Glycosaminoglycan Sulfation and Signaling

Glycosaminoglycans are large linear polysaccharides constructed of repeating disaccharide units.

Protocolli

Enzymatic Assay of Hyaluronidase (3.2.1.35)

This procedure may be used for Hyaluronidase products.

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