H2126

Hyaluronidase from sheep testes

Type II, lyophilized powder, ≥300 units/mg solid

• Sinonimo/i: Hyaluronate 4-glycanohydrolase, Hyaluronoglucosaminidase
• Numero CAS: 37326-33-3
• Classificazione EC (Enzyme Commission): 3.2.1.35 (BRENDA, IUBMB)
• Numero CE: 253-464-3
• Numero MDL: MFCD00131351
• Codice UNSPSC: 12352204
• NACRES: NA.54

Proprietà

• Coniugato: conjugate (Glucosaminoglycan)
• Livello qualitativo: 200
• Tipo: Type II
• Forma fisica: lyophilized powder
• Attività specifica: ≥300 units/mg solid
• PM: 55 kDa
• Temperatura di conservazione: −20°C

Descrizione

Descrizione generale

Hyaluronidase is a hyaluronic acid-metabolizing enzyme. It is found in the venoms of snake, honeybee and hymenoptera. Hyaluronidase degrades hyaluronic acid (HA), which enhances membrane permeability, lowers viscosity and increases tissue permeability. It enhances the diffusion of local anesthetics, lowers the size of myocardial infarction, improves the effects of anticancer drugs and prevents perineal trauma during spontaneous delivery.

Applicazioni

Hyaluronidase from sheep testes has been used:

• in flow cytometry
• in the isolation of putative spermatogonial stem cells (SSCs)
• to study the effects of magnetic stimulation in magnetic hydrogel properties
• in immunohistochemistry to visualize collagen type II distribution
• to expose group-III auditory neurons of Muller′s organ for patch-clamp recordings
• for the degeneration of hydrogels

Azioni biochim/fisiol

These enzymes randomly cleave β-N-acetylhexosamine-(1−4)-glycosidic bonds in hyaluronic acid, chondroitin, and chondroitin sulfates.

Definizione di unità

One unit will degrade 0.75 μg of the polysaccharide hyaluronic acid per minute at pH 5.35 at 37 ºC (as measured by turbidimetric absorbance (λ: 600nm) when complexed with BSA after 45 minutes).

Stato fisico

Lyophilized powder containing lactose

Altre note

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H334
• Consigli di prudenza: P261 - P284 - P501
• Classi di pericolo: Resp. Sens. 1
• Codice della classe di stoccaggio: 11 - Combustible Solids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)