GE28-9365-52
HiPrep™ IMAC Fast Flow 16/10
Cytiva 28-9365-52
About This Item
Prodotti consigliati
Confezionamento
pkg of 1 ea
Produttore/marchio commerciale
Cytiva 28-9365-52
Parametri
1.5 bar (22 psi) (Over the Packed Bed During Operation)
22 psi
Volume del letto
20 mL
Diam. int. colonna
16 mm
Matrice
6% cross-linked agarose
Dimensione particelle
45-165 μm
Diametro medio
90 μm
cleaning
2-14(Medium uncharged with metal ions.)
working range
3-12
Capacità
~40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Compatibilità
suitable for bioprocess medium
Categorie correlate
Descrizione generale
Applicazioni
IMAC Sepharose™ 6 Fast FLow consists of 90 ?m beads of highly cross-linked Agarose to which a chelating group has been covalently coupled. This chelating group can be charged with suitable metal ions, specific for your target Protein of interest. The bead size of IMAC Sepharose™ 6 Fast FLow enables high flow properties, making the medium an excellent choice for scaling up purification at laboratory scale and purification of histidine-tagged Proteins at process scale. Low metal ion leakage means that the activity of the purified Protein is retained and the risk of precipitation reduced, which results in increased purity, activity, and yield of the target Protein. Leakage of metal ions into the eluted target Protein pool from IMAC Sepharose™ 6 Fast FLow is generally Low under normal conditions.
IMAC Sepharose™ 6 Fast FLow is highly stable and compatible with a wide range of common additives. This helps maintain biological activity and increase product yield, while at the same time greatly expanding the range of suitable operating conditions.
The medium is available prepacked in HiTrap® HiScreen™ and HiPrep™ column formats for convenient purification of histidine-tagged Proteins at laboratory scale. Initial purification using a 1 mL HiTrap® IMAC FF column can be scaled up further with the 5 mL column. Purification can be scaled up even more using 20 mL HiPrep™ IMAC FF 16/10 columns. Prepacked HiScreen™ IMAC FF with a 10 cm bed height is the format of choice for method optimization and process development.
Caratteristiche e vantaggi
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion.
- Conveniently charge with your metal of choice.
- Prepacked 20 mL HiPrep™ columns for easy scale-up.
- High binding capacity.
Stoccaggio e stabilità
Risultati analitici
Altre note
Note legali
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Prodotti correlati
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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Articoli
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
This page shows troubleshooting instructions for affinity chromatography of tagged proteins using GE Healthcare products.
This page shows the characteristics of Ni Sepharose, Ni Sepharose excel, TALON Superflow, and uncharged IMAC Sepharose products from GE Healthcare.
Protocolli
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
This page covers detailed information on cleaning procedures and recommended flow for column cleaning.
This page shows how to perform sample desalting, buffer exchange and concentration for affinity chromatography of tagged proteins.
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