GE17-5255-01

HisTrap^™ Fast Flow

Cytiva 17-5255-01, pack of 5 × 5 mL

• Sinonimo/i: HisTrap FF, His-tagged protein purification column, ready-to-use column
• Codice UNSPSC: 23151816
• NACRES: NA.56

Proprietà

• Confezionamento: pack of 5 × 5 mL
• Produttore/marchio commerciale: Cytiva 17-5255-01
• Parametri: <4 mL/min flow rate; 42 psi
• Dimensioni del letto: 7 mm × 25 mm
• Volume del letto: 1 mL
• Diam. int. colonna: 7 mm
• Matrice: 6% cross-linked agarose
• Dimensione particelle: 45-165 μm
• Diametro medio: 90 μm
• cleaning: 2-14(Ni²⁺-stripped medium.)
• working range: 3-12(Ni²⁺-stripped medium.)
• Capacità: ~40 mg binding capacity(histidine-tagged protein)
• Compatibilità: suitable for bioprocess medium

Descrizione

Descrizione generale

HisTrap^™ FF is a ready-to-use column, prepacked with precharged Ni Sepharose^™ 6 Fast Flow for preparative purification of histidine-tagged recombinant proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose^™ 6 Fast Flow consists of 90 μm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized. This chelating ligand is charged with Ni²⁺ ions, the first-choice metal ion for purifying most histidine-tagged proteins. The negligible leakage of Ni²⁺ ions from the matrix ensures reliable capture of histidine-tagged proteins in repeated IMAC purifications.

The high flow rates made possible by the Ni Sepharose^™ 6 Fast Flow matrix make HisTrap^™ FF columns well suited for small scale-up. Capacity can be increased by connecting columns in series. HiTrap^® IMAC FF is the product of choice when charging the medium with different metal ions for optimization of purification protocols.

Caratteristiche e vantaggi

• High binding capacity, approx. 40 mg/mL medium.
• Negligible leakage of Ni²⁺.
• Prepacked columns offer reliable and convenient time-saving purification of histidinetagged recombinant proteins.
• Compatible with a wide range of reducing agents, detergents, denaturants, and other additives.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

HiTrap is a registered trademark of Cytiva

HisTrap is a trademark of Cytiva

Sepharose is a trademark of Cytiva

Informazioni sulla sicurezza

• Pittogrammi: GHS09
• Avvertenze: Warning
• Indicazioni di pericolo: H226
• Consigli di prudenza: P280 - P210 - P241 - P235 - P303 + P361 + P353 - P501
• Codice della classe di stoccaggio: 3 - Flammable liquids