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GE17-5144-01

HiTrap® Benzamidine Fast Flow

Cytiva 17-5144-01, pack of 1 × 5 mL

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About This Item

Codice UNSPSC:
23151816
NACRES:
NA.56

ligand

p-aminobenzamidine

Confezionamento

pack of 1 × 5 mL

Produttore/marchio commerciale

Cytiva 17-5144-01

Condizioni di stoccaggio

(0.05 M Sodium Acetate, pH 4.0 in 20% Ethanol)

Parametri

42 psi

Dimensioni del letto

16 mm × 25 mm

Volume del letto

5 mL

Diam. int. colonna

16 mm

Matrice

highly cross-linked 4% agarose

Diametro medio

90 μm

cleaning

1-9

Intervallo di esercizio

2-8

Temperatura di conservazione

2-8°C

Categorie correlate

Descrizione generale

HiTrap® Benzamidine FF (high sub) are prepacked with Benzamidine Sepharose™ 4 Fast FLow (high sub) and designed specifically for the removal and/or purification of serine proteases such as thrombin.

Applicazioni

HiTrap® Benzamidine FF (high sub) are prepacked 1 mL and 5 mL columns for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein. Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.

The medium, Benzamidine Sepharose™ 4 Fast FLow (high sub), is also available as lab packages and is an excellent choice for scale-up. The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTAdesign or FPLC™ System.

Caratteristiche e vantaggi

  • Effective removal of thrombin and factor Xa after tag cleavage of recombinant Proteins.
  • Removal of serine proteases is easily done directly from serum, monoclonal cell supernatants and bacterial lysates.
  • HiTrap® Benzamidine FF for convenient, one-step removal and/or purification of trypsin, trypsin-like serine proteases, and zymogens including urokinase and prekallikrein.
  • High binding capacity.
  • The columns can be operated using syringe, peristaltic pump or liquid chromatography system such as ÄKTA® design or FPLC™ System.

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Altre note

The pressure over the packed bed varies depending on a range of parameters such as the characteristics of the chromatography medium and the column tubing used.

Note legali

FPLC is a trademark of Cytiva
HiTrap is a registered trademark of Cytiva
Sepharose is a trademark of Cytiva
ÄKTA is a registered trademark of Cytiva

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Flame
GHS02

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

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Certificati d'analisi (COA)

Lot/Batch Number

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Articoli

Principles and standard conditions for different purification techniques

This page provides information about the principles and standard conditions for different purification techniques for the purification of multiprotein complexes with from GE Healthcare.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using GE Healthcare products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from GE Healthcare.

Cleavage and Purification of GST-Tagged Protein Bound to GSTrap

This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from GE Healthcare.

Vedi tutto

Protocolli

Removal of Thrombin and Factor Xa Using HiTrap® Benzamidine FF (High Sub)

This page shows how to remove thrombin and Factor Xa using HiTrap® Benzamidine FF (high sub) from GE Healthcare.

Removal of GST Tag by Enzymatic Cleavage

This page how to remove GST tags by enzymatic cleavage using GE Healthcare products.

Performing a Separation with GE Healthcare Products Based on GST Fusion Proteins

How to perform a separation with GST fusion proteins which are affinity chromatography tags used in GST MicroSpin Purifcation Module, GSTrap FF, GSTPrep FF 16/10, Glutathione Sepharose 4 Fast Flow and Glutathione Sepharose 4B products from GE Healthcare.

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