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Key Documents

GE28-9889-49

Sigma-Aldrich

XK 26/40 Column

Sinonimo/i:

XK Column

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About This Item

Codice UNSPSC:
41115711
NACRES:
SB.52

tecniche

LPLC: suitable

Descrizione generale

XK empty columns provide reliable and reproducible separations. The tube material is of borosilicate glass, which allows for visual inspection of the media bed. The columns are made of biocompatible materials that exhibit excellent chemical resistance. A broad range of column dimensions makes XK columns suitable for many different purification needs. Column packing can be performed using either a packing reservoir or an extra column tube attached with a packing connector. The QuickLock mechanism of the adapter shaft facilitates rapid and easy movement of the adapter, simplifying adjustments of the bed height, disassembly, and cleaning. The design of the adapter plunger gives a uniform flow, which maintains the integrity of the packed bed during operations. An extensive range of spare parts is available, facilitating maintenance.

Caratteristiche e vantaggi

Adapter QuickLock mechanism that facilitates column handling and cleaning
Cooling jacket for sensitive purifications
Fittings for direct connection to chromatographiy systems
Plunger design to ensure a homogeneous buffer distribution and reproducible packing
User friendly and robust empty columns, designed for liquid chromatography at low to medium pressure.

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Protocolli

This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.

Here we discuss purification options when working with Sepharose High Performance and we present some examples of media screening, capture and purification.

This page shows how to purify histidine-tagged proteins using TALON Superflow from GE Healthcare.

This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from GE Healthcare.

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