GE17-0920-07
IMAC Sepharose™ High Performance
Cytiva 17-0920-07, pack of 100 mL
About This Item
Prodotti consigliati
Confezionamento
pack of 100 mL
Produttore/marchio commerciale
Cytiva 17-0920-07
Matrice
highly cross-linked 6% agarose
Dimens. media delle particelle
34 μm
cleaning in place
2-14(Medium uncharged with metal ions.)
working range
3-12
Capacità
≥40 mg binding capacity (histidine-tagged protein/ml medium when changed with Ni2+)(Protein- and metal-ion dependent.)
Descrizione generale
Applicazioni
Caratteristiche e vantaggi
- For optimizing purification of histidine-tagged Proteins when Ni2+ is not the best choice of metal ion
- Conveniently charge with your metal of choice
- 34 μm bead size for high performance purification
- High binding capacity
Stoccaggio e stabilità
Risultati analitici
Note legali
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Avvertenze
Warning
Indicazioni di pericolo
Consigli di prudenza
Codice della classe di stoccaggio
3 - Flammable liquids
Certificati d'analisi (COA)
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Articoli
This page shows how to separate proteins and peptides with affinity for metal ions by immobilized metal chelate affinity chromatography using HiTrap Chelating HP, Chelating Sepharose Fast Flow,His MicroSpin Purification Module or HisTrap Kit from GE Healthcare.
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.
This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.
This page shows how to optimize purification of histidine-tagged proteins using GE Healthcare products.
Protocolli
Purification of Histidine-Tagged Proteins using IMAC Sepharose® High Performance
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap Kit, HiTrap Chelating HP and Chelating Sepharose Fast Flow products from GE Healthcare.
Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.
Sample Preparation for Affinity Chromatography in Specific Groups of Biomolecules
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