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GE17-5268-01

Ni Sepharose™ High Performance

Cytiva 17-5268-01, pack of 25 mL

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About This Item

Codice UNSPSC:
41106500
NACRES:
NA.56

Confezionamento

pack of 25 mL

Produttore/marchio commerciale

Cytiva 17-5268-01

Matrice

highly cross-linked 6% agarose

Dimens. media delle particelle

34 μm

cleaning in place

2-14(Ni2+-stripped medium.)

working range

3-12(Ni²⁺-stripped medium.)

Capacità

≥40 mg binding capacity (histidine-tagged protein/ml medium)(Protein binding capacity is protein-to-protein dependent.)

Descrizione generale

Ni Sepharose™ High Performance affinity media for high-resolution, high capacity purification of histidine-tagged proteins by immobilized metal ion affinity chromatography (IMAC).

Ni Sepharose™ High Performance consists of highly cross-linked agarose beads to which a chelating group has been coupled. This chelating group is precharged with Ni2+, which will selectively retain proteins with exposed histidine groups.

The high stability and broad compatibility of Ni Sepharose™ High Performance maintains biological activity and increases product yield, at the same time as it greatly expands the range of suitable operating conditions.

Caratteristiche e vantaggi

  • High-performance purification of histidine-tagged proteins
  • Negligible leakage of Ni2+ ions.
  • High binding capacity, at least 40 mg/mL medium
  • Compatible with a wide range of reducing agents, detergents, denaturants and other additives
  • 34 μm bead size for improved resolution

Stoccaggio e stabilità

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol)

Risultati analitici

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Note legali

IMAC Sepharose products, Ni Sepharose products and Fe Sepharose products
These products are sold under a license from Sigma-Aldrich under patent number EP 1276716 (Metal chelating compositions) and equivalent patents and patent applications in other countries.
Sepharose is a trademark of Cytiva

Pittogrammi

Environment
GHS09

Avvertenze

Warning

Indicazioni di pericolo

H226

Codice della classe di stoccaggio

3 - Flammable liquids

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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Articoli

Purification or Removal of Proteins and Peptides

This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from GE Healthcare.

Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates

This page shows high-throughput screening using His MultiTrap HP and His MultiTrap FF 96-well filter plates from GE Healthcare.

Performing a Cell Lysis of Recombinant Histidine-Tagged Proteins

This page shows a general procedure for cell lysis of recombinant histidine-tagged proteins using GE Healthcare products.

Protocolli

Minipreps using His SpinTrap™ and His SpinTrap™ Kit

This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from GE Healthcare.

Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose® High Performance

This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose High Performance from GE Healthcare.

Purification of Protein A-Tagged Proteins

This page shows how to purify protein A-tagged proteins using GE Healthcare products.

Column Packing and Preparation for Affinity Chromatography with Specific Groups of Biomolecules

Column packing and preparation for Affinity Chromatography with specific groups of biomolecules.

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