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Key Documents

Altri documenti

DUO92106

Sigma-Aldrich

Duolink® In Situ Orange Starter Kit Goat/Rabbit

Sinonimo/i:

in situ Proximity Ligation Assay Kit, Protein Protein Interaction Kit

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About This Item

Codice UNSPSC:
41116158
NACRES:
NA.32

Nome Commerciale

Duolink®

tecniche

proximity ligation assay: suitable

Fluorescenza

λex 554 nm; λem 576 nm (orange) (Cyanine 3; Zeiss Filter set 20)

Compatibilità

suitable for fluorescence

Temperatura di conservazione

−20°C

Applicazioni

Duolink®proximity ligation assay(PLA®) allows for endogenous detection of protein interactions, post translational modifications, and protein expression levels at the single molecule level in fixed cells and tissue samples.

Follow the Duolink® In Situ Fluorescence Protocol to use this product. A set of short instructionsis also available.

Visit our Duolink® PLA Resource Center for information on how to run a Duolink® experiment, applications, troubleshooting, and more.

To perform a complete Duolink® PLA in situ experiment you will need two primary antibodies (PLA, IHC, ICC or IF validated) that recognize two target epitopes.This starter kit supplies all other necessary reagents for 30 Duolink® PLA reactions, which include a pair of PLA probes (Anti-Goat PLUS and Anti-Rabbit MINUS), orange detection reagents, wash buffers, and mounting medium. Note that the primary antibodies must come from the same species as the Duolink® PLA probes. Analysis is carried out using standard immunofluorescence assay equipment.
Specificity
The Duolink® PLA in situ reagents are suitable for use on fixed cells, cytospin cells, cells grown on slide, formalin-fixed, paraffin embedded (FFPE), or tissue (fresh or frozen). No minimum number of cells is required.

Let us do the work for you, learn more about our Custom Service Program to accelerate your Duolink® projects

View full Duolink® product list

Caratteristiche e vantaggi

  • No overexpression or genetic manipulation required
  • High specificity (fewer false positives)
  • Single molecule sensitivity due to rolling circle amplification
  • Relative quantification possible
  • No special equipment needed
  • Quicker and simpler than FRET
  • Increased accuracy compared to co-IP
  • Publication-ready results

Note legali

Duolink is a registered trademark of Merck KGaA, Darmstadt, Germany
PLA is a registered trademark of Merck KGaA, Darmstadt, Germany

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • DUO92005Duolink® In Situ PLA® Probe Anti-Rabbit MINUSSDS
  • DUO92003Duolink® In Situ PLA® Probe Anti-Goat PLUSSDS
  • DUO92007Duolink® In Situ Detection Reagents OrangeSDS
  • DUO82049Duolink® In Situ Wash Buffers, FluorescenceSDS
  • DUO82040Duolink® In Situ Mounting Medium with DAPISDS

Pittogrammi

Health hazardCorrosion
GHS08,GHS05

Avvertenze

Danger

Indicazioni di pericolo

H290,H334,H412

Classi di pericolo

Aquatic Chronic 3 - Met. Corr. 1 - Resp. Sens. 1

Codice della classe di stoccaggio

8A - Combustible corrosive hazardous materials

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

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Thrombomodulin promotes focal adhesion kinase activation and contributes to angiogenesis by binding to fibronectin
Ysu YY, et al.
Oncotarget, 7(42), 68122-68139 (2016)
Phosphorylation-mediated interactions with TOPBP1 couple
2 53BP1 and 9-1-1 to control the G1 DNA damage checkpoint
Pearl LH, et al.
bioRxiv, 510032-510032 (2019)
Shaida Ouladan et al.
International journal of oncology, 46(6), 2595-2605 (2015-04-23)
Solitary fibrous tumors (SFTs) are rare mesenchymal neoplasms, displaying variable morphological and clinicopathological features. Supportive immunohistochemical markers such as CD34, CD99, BCL2 and LSD1 are commonly applied in the differential diagnosis of SFTs, although none is sufficiently sensitive or specific
Gunisha Sagar et al.
Gut, 65(7), 1165-1174 (2015-06-11)
New-onset diabetes and concomitant weight loss occurring several months before the clinical presentation of pancreatic cancer (PC) appear to be paraneoplastic phenomena caused by tumour-secreted products. Our recent findings have shown exosomal adrenomedullin (AM) is important in development of diabetes
Dipti Pravin Lambade et al.
Journal of clinical and diagnostic research : JCDR, 9(2), ZC01-ZC05 (2015-04-11)
The purpose of this vitro study was to comparatively evaluate the adhesive bonding of dual cured resin luting agents with lithium disilicate ceramic material. Porcelain laminate veneers were prepared with lithium disilicate ceramic material i.e. IPS Empress II( E-Max Press).
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Articoli

Duolink® PLA Technology for Neuroscience Applications

Duolink proximity ligation assay used to study neuron interactions furthering neuroscience research.

Duolink® PLA Fluorescence Protocol

Protocol for immunofluorescent detection of proteins in cells and tissue

Duolink® References

Find Duolink references based on the type of method used, post translational modification detected, and research focus.

How to Optimize the Duolink® Proximity Ligation Assay

Things to consider for preparation, setup and execution of the Duolink® assay protocol

Vedi tutto

Protocolli

Duolink® In Situ Short Instructions - Fluorescence

This page details the Duolink® In Situ Short Protocol for fluorescence detection

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