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CY0100

Sigma-Aldrich

Cytochrome c Reductase (NADPH) Assay Kit

1 kit sufficient for 100 tests, determining cytochrome c reductase activity

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

Livello qualitativo

200

impiego

 kit sufficient for 100 tests

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... POR(5447)

Categorie correlate

Azioni biochim/fisiol

Eukaroytic NADPH-cytochrome c reductase (NADPH-cytochrome P450 reductase, EC1.6.2.4) is a flavoprotein localized to the endoplasmic reticulum. It transfers electrons from NADPH to several oxygenases, the most important of which are the cytochrome P450 family of enzymes, responsible for xenobiotic detoxification. NADPH-cytochrome c reductase is widely used as an endoplasmic reticulum marker and as one of the biomarkers for ecological pollution and dietary lipid uptake.

Caratteristiche e vantaggi

  • Detection of endoplasmic reticulum (ER) during isolation and subcellular fractionation by density gradient separation
  • Colorimetric measurement of NADPH-Cytochrome C Reductase activity in various sources after intoxication by drugs or challenge by xenobiotics
  • Analyze enrichment and function of ER

Risultati analitici

The Cytochrome C Reductase Assay Kit contains all the reagents required for a fast and convenient determination of NADPH-Cytochrome C Reductase activity in cell and tissue extracts and in purified microsomes. It has been tested on samples prepared from various species of mammalian tissues such as liver, kidney, brain, spleen, and heart muscle, as well as on lysates from cell lines such as HeLa, HepG2, and Jurkat. In addition, it has been tested on samples prepared from the yeast strains P. pastoris and S. cerevisiae.

Solo come componenti del kit

N° Catalogo
Descrizione
  • Assay Buffer 100 mL
  • Enzyme Dilution Buffer 25 mL
  • Cytochrome c 50 mg
  • Cytochrome c Reductase (NADPH) Positive Control from rabbit liver 50 μL
  • Cytochrome c Oxidase Inhibitor Solution 1 mL

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • N6505β-Nicotinamide adenine dinucleotide 2′-phosphate reduced tetrasodium salt hydrate, ≥95% (HPLC) 25 mgSDS

Prodotti correlati

N° Catalogo
Descrizione
Determinazione del prezzo

Pittogrammi

Skull and crossbones
GHS06

Avvertenze

Danger

Indicazioni di pericolo

H301,H412

Classi di pericolo

Acute Tox. 3 Oral - Aquatic Chronic 3

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

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Certificati d'analisi (COA)

Lot/Batch Number
SLBW8945
127M4111V
11170731
047M4072V
11170229

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Rohit Sharma et al.
Age (Dordrecht, Netherlands), 36(4), 9686-9686 (2014-07-20)
Imbalance in Th1/Th2 immune pathways and cellular antioxidant systems with progressive aging are among the leading causes of increased risk of morbidity and mortality in elderly. Although probiotics have been considered to boost immune system, there is a lack of
Nathan M Long et al.
Reproductive biology and endocrinology : RB&E, 11, 34-34 (2013-05-10)
Previously we reported decreased circulating progesterone and fertility in one and two year old ewes born to undernourished mothers. This study was designed to investigate if this reduction in progesterone persisted into old age, and if it did, what mechanisms
D Plonne et al.
Analytical biochemistry, 276(1), 88-96 (1999-12-10)
A novel method is described for the separation on a single gradient of the major intracellular organelles of the secretory pathway, the Golgi, the smooth endoplasmic reticulum, and the rough endoplasmic reticulum. Total microsomes were prepared from rat liver by
Amanda Spring de Almeida et al.
Life sciences, 276, 119469-119469 (2021-04-04)
Breast cancer-induced chronic pain is usually treated with opioids, but these compounds cause various adverse effects. Transient receptor potential ankyrin 1 (TRPA1) is involved in cancer pain; also, endogenous TRPA1 agonists are associated with cancer pain development. The aim of
C O Lemley et al.
Journal of dairy science, 93(3), 1012-1021 (2010-02-23)
In the cow, inadequate concentrations of progesterone during gestation may lead to an abrupt termination of pregnancy. The primary organ involved in progesterone catabolism is the liver, which contains an abundance of cytochrome P450 isozymes (EC 1.14.14.1; mixed-function monooxygenases). The
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