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CYTOCOX1

Sigma-Aldrich

Cytochrome c Oxidase Assay Kit

sufficient for 100 tests, soluble and membrane bound mitochondria

Sinonimo/i:

Cytochrome c Oxidase Activity Assay Kit

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

Livello qualitativo

200

impiego

sufficient for 100 tests

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... COX1(4512)

Applicazioni

The Cytochrome c Oxidase Assay Kit uses an optimized colorimetric assay based on observation of the decrease in absorbance of ferrocytochrome c measured at 550 nm, which is caused by its oxidation to ferricytochrome c by cytochrome c oxidase. This kit is suitable for the detection of mitochondrial outer membrane integrity/mitochondrial stress and for the detection of mitochondria in subcellular fractions.

Azioni biochim/fisiol

Cytochrome c oxidase [EC 1.9.3.1] is located on the inner mitochondrial membrane dividing the mitochondrial matrix from the intermembrane space, and has traditionally been used as a marker for this membrane. It is also located in the cytoplasmic membrane of bacteria. Cytochrome c oxidase provides energy for the cell by coupling electron transport through the cytochrome chain with the process of oxidative phosphorylation.

Caratteristiche e vantaggi

  • Simple, optimized protocol - Obtain reproducible results without special training needs
  • Useful for determining cytochrome c activity from any mitchondrial source - The enzyme is present in all mitochondria regardless of species
  • Useful for detecting the presence of mitochondria in subcellular fraction - Save time and increase confidence in the quality of organelle preparations
  • May be used in conjunction with the MITOISO1 Mitochondrial Isolation Kit - Standardized mitochondrial preparation and analysis ensures reproducibility
  • Use to analyze the intactness of mitochondrial membranes
  • Simple colorimetric measurement of solution

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • C2506Cytochrome c from equine heart, ≥95% (SDS-PAGE) 50 mgSDS
  • D4641n-Dodecyl β-D-maltoside, ≥98% (GC) 10 mgSDS

Pittogrammi

Corrosion
GHS05

Avvertenze

Danger

Indicazioni di pericolo

H315,H318

Classi di pericolo

Eye Dam. 1 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
079M4190V
11190179
058M4121V
11180208
11170506

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PloS one, 6(12), e28536-e28536 (2011-12-24)
Human primary myotubes are highly glycolytic when cultured in high glucose medium rendering it difficult to study mitochondrial dysfunction. Galactose is known to enhance mitochondrial metabolism and could be an excellent model to study mitochondrial dysfunction in human primary myotubes.
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The habenula is a nuclear complex composed of the lateral habenula (LHb) and medial habenula (MHb), two distinct structures. Much progress has been made to emphasize the role of the LHb in the pathogenesis of depression. In contrast, relatively less
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