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MITOISO2

Sigma-Aldrich

Mitochondria Isolation Kit

sufficient for 50 applications (2-5 x 107 cells), isolation of enriched mitochondrial fraction from cells

Sinonimo/i:

Mitochondrial Extraction Kit, Mitochondrial Purification Kit

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About This Item

Codice UNSPSC:
12352200
NACRES:
NA.32

Livello qualitativo

200

impiego

sufficient for 50 applications (2-5 x 107 cells)

tecniche

ELISA: suitable
western blot: suitable

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

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Descrizione generale

Mitochondria, a double membrane structure, is the site of most energy production in eukaryotic cells. The mitochondria isolation kit contains two buffers: one for extracting proteins for functional studies and another for profiling in proteome studies. Additionally, the kit includes a storage buffer for preserving intact mitochondria.

Applicazioni

Mitochondria Isolation Kit has been used in the isolation of mitochondria from:
  • Ewing′s Sarcoma Family of Tumor (ESFT) cells
  • blood samples of tuberculosis patients
  • human neuroblastoma cells and glioma cells
  • myotubes
  • HepG2 cells

Caratteristiche e vantaggi

  • The kit allows for quick and simple extraction of a concentrated portion of mitochondria from cells.
  • The isolated mitochondria will mostly have intact inner and outer membranes.
  • Can be used to extract mitochondrial proteins for proteome analysis

Altre note

  • This product is for R&D use only, not for drug, household, or other uses.
  • Please consult the Material Safety Data Sheet for information regarding hazards and safe handling practices.
  • Use ultrapure water for the preparation of reagents.
  • Please refer to the product information sheet to learn more about kit components, reagents required, storage and stability, and isolation protocols.

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • C2978CelLytic™ M, Cell Lysis Reagent, Suitable for Mammalian cell lysis and protein solubilization. 10 mLSDS
  • P8340Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution 2.5 mLSDS
  • C0356Protein Extraction Reagent Type 4 1 bottleSDS

Avvertenze

Warning

Indicazioni di pericolo

H302,H315,H319,H351,H361fd,H411

Classi di pericolo

Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2 - Repr. 2 - Skin Irrit. 2

Codice della classe di stoccaggio

10 - Combustible liquids

Punto d’infiammabilità (°F)

188.6 °F - closed cup

Punto d’infiammabilità (°C)

87 °C - closed cup

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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