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CASP3C

Sigma-Aldrich

Caspase 3 Assay Kit, Colorimetric

Sinonimo/i:

Colorimetric Caspase-3 Activity Assay Kit

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About This Item

Codice UNSPSC:
12161503
NACRES:
NA.84

impiego

sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
 sufficient for 100 standard tests (1 ml)

Livello qualitativo

300

Condizioni di spedizione

dry ice

Temperatura di conservazione

−20°C

Informazioni sul gene

human ... CASP3(836)

Descrizione generale

Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.

The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).

Applicazioni

The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.

Definizione di unità

One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.

Altre note

Use 17 megohm water only

Solo come componenti del kit

N° Catalogo
Descrizione
  • Water (17-18 megaOhm) 125 mL

I componenti del kit sono disponibili anche separatamente

N° Catalogo
Descrizione
SDS
  • A0835Ac-DEVD-CHO inhibitor .5 mgSDS
  • A2559Ac-DEVD-pNA substrate 15 mgSDS
  • p-Nitroaniline standard 1 mg

Pittogrammi

Skull and crossbonesHealth hazard
GHS06,GHS08

Avvertenze

Danger

Indicazioni di pericolo

H301 + H311 + H331,H373,H412

Classi di pericolo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2

Codice della classe di stoccaggio

6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

Punto d’infiammabilità (°F)

415.4 °F - closed cup

Punto d’infiammabilità (°C)

213 °C - closed cup

Certificati d'analisi (COA)

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