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A7058

Sigma-Aldrich

Anti-polyHistidine−Peroxidase antibody, Mouse monoclonal

clone HIS-1, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-polyHistidine, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.56

Origine biologica

mouse

Livello qualitativo

200

Coniugato

peroxidase conjugate

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

HIS-1, monoclonal

Forma fisica

lyophilized powder

Confezionamento

vial of 0.5 mL

Concentrazione

5-11 mg/mL

tecniche

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

Isotipo

IgG2a

Temperatura di conservazione

2-8°C

modifica post-traduzionali bersaglio

unmodified

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Categorie correlate

Descrizione generale

The monoclonal anti-polyHistidine Peroxidase Conjugate antibody recognizes native and denatured forms of synthetic polyhistidine or polyhistidine-tagged fusion proteins. The product is reactive with fusion proteins expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Specificità

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Immunogeno

recombinant polyHistidine tagged fusion protein.

Applicazioni

Antibody suitable for immunoblotting. Working dilution 1:20



Also suitable for dot blot assays and ELISA

Stato fisico

Lyophilized from 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 0.05% MIT.

Nota sulla preparazione

Prepared by the two-step glutaraldehyde method described by Avrameas, S., et al., Scand. J. Immunol., 8, Suppl. 7, 7 (1978).

Ricostituzione

The antibody conjugate should be reconstituted with 0.5 ml deionized water.

Altre note

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

Note legali

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Pittogrammi

Exclamation mark
GHS07

Avvertenze

Warning

Indicazioni di pericolo

H317

Classi di pericolo

Skin Sens. 1

Codice della classe di stoccaggio

12 - Non Combustible Liquids

Classe di pericolosità dell'acqua (WGK)

WGK 2

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Certificati d'analisi (COA)

Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.

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