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A5588

Sigma-Aldrich

Anti-polyHistidine−Alkaline Phosphatase antibody, Mouse monoclonal

clone HIS-1, purified from hybridoma cell culture

Sinonimo/i:

Monoclonal Anti-polyHistidine, Monoclonal Anti-polyHistidine−Alkaline Phosphatase antibody produced in mouse, Monoclonal 6 His epitope tag, Monoclonal 6xHis-tag, Monoclonal HHHHHH epitope tag, Monoclonal Hexa His tag, Monoclonal His-tag, Monoclonal His6 tag, Monoclonal Histidine tagged, Monoclonal Poly-His-tag

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About This Item

Codice UNSPSC:
12352203
NACRES:
NA.56

Origine biologica

mouse

Coniugato

alkaline phosphatase conjugate

Forma dell’anticorpo

purified from hybridoma cell culture

Tipo di anticorpo

primary antibodies

Clone

HIS-1, monoclonal

Stato

buffered aqueous glycerol solution

tecniche

western blot: 1:2,000 using lysates of Escherichia coli induced to express a 6xHis tagged protein

Isotipo

IgG2a

Condizioni di spedizione

wet ice

Temperatura di conservazione

2-8°C

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Descrizione generale

The Monoclonal Anti-polyHistidine-Alkaline Phosphatase antibody recognizes native and denatured-reduced forms of synthetic polyhistidine or polyhistidine tagged fusion proteins. The product is reactive with fusion protein expressed by prokaryotic pET, pRSET and pTrc expression vectors.

Specificità

The antibody recognizes synthetic polyHistidine, as well as native or denatured, reduced forms of proteins tagged with 6X histidines, expressed in selected vectors.

Immunogeno

recombinant polyHistidine tagged fusion protein.

Applicazioni

Antibody is suitable for ELISA, immunoblotting and dot blotting assays.

Stato fisico

Solution in 0.05 M Tris buffer, pH 8.0, containing 1 mM MgCl2, 1% bovine serum albumin, 50% glycerol and 15 mM sodium azide

Note legali

This Product is covered by patent DE 19507166 and foreign equivalents exclusively licensed to QIAGEN GmbH, Qiagen Strasse 1, D-40724 Hilden, Germany.

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Codice della classe di stoccaggio

10 - Combustible liquids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

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Certificati d'analisi (COA)

Lot/Batch Number
108M4773V
21180563
127M4849V
21171021
096M4841V

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