94310

Uricase from Bacillus fastidiosus

lyophilized, ~9 U/mg

• Sinonimo/i: Urate oxidase
• Numero CAS: 9002-12-4
• Classificazione EC (Enzyme Commission): 1.7.3.3 (BRENDA, IUBMB)
• Numero MDL: MFCD00082126
• Codice UNSPSC: 12352204
• ID PubChem: 57653596
• NACRES: NA.54

Proprietà

• Origine biologica: Bacillus sp. (B. fastidiosus)
• Forma fisica: powder
• Qualità: lyophilized
• Attività specifica: ~9 U/mg
• Condizioni di stoccaggio: dry at room temperature
• Concentrazione: 90—100%
• Colore: white to faint beige
• Solubilità: 5 mg/mL, clear, colorless to very faintly yellow (in enzyme dilution buffer)
• applicazioni: detection
• Temperatura di conservazione: −20°C
• Stringa SMILE: N.OCC1OC(C(O)C1OP(O)(=O)OCC2OC(C(O)C2O)N3C=CC(=O)NC3=O)N4C=CC(=O)NC4=O
• InChI: 1S/C18H23N4O14P.H3N/c23-5-7-14(13(28)16(34-7)22-4-2-10(25)20-18(22)30)36-37(31,32)33-6-8-11(26)12(27)15(35-8)21-3-1-9(24)19-17(21)29;/h1-4,7-8,11-16,23,26-28H,5-6H2,(H,31,32)(H,19,24,29)(H,20,25,30);1H3; VPXDHZMLJOJKOX-UHFFFAOYSA-N

Descrizione

Descrizione generale

Research area: Cell signaling

Uricase is present in many organisms, such as, archaea, bacteria, and eukaryotes. The Gut microbiome is the major source of uricase, a homotetrameric enzyme containing four identical 34 kDa subunits.

Applicazioni

Uricase may be used for the determination of uric acid in biological fluids. It may be helpful in the spectrophotometric determination of purines and purine nucleosides.

Azioni biochim/fisiol

Uricase catalyzes the oxidation of uric acid to 5-hydroxyisourate, which subsequently forms (S)-allantoin. It is involved in purine metabolism, urate degradation and (S)-allantoin formation The absence of functional uricase in humans has significant physiological and pathophysiological implications. It has been associated with higher serum uric acid levels, leading to an increased risk of conditions related to uric acid crystallization, such as gout. Uricase has emerged as an important therapeutic target for the treatment of conditions related to hyperuricemia, such as gout.

Definizione di unità

1 U corresponds to the amount of enzyme which oxidizes 1 μmole uric acid at pH 9.1 and 37 °C.

Altre note

For the determination of uric acid in biological fluids; In the spectrophotometric determination of purines and purine nucleosides.

Informazioni sulla sicurezza

• Pittogrammi: GHS08
• Avvertenze: Danger
• Indicazioni di pericolo: H319,H334,H360FD
• Consigli di prudenza: P202 - P261 - P264 - P280 - P305 + P351 + P338 - P308 + P313
• Classi di pericolo: Eye Irrit. 2 - Repr. 1B - Resp. Sens. 1
• Codice della classe di stoccaggio: 6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable
• Dispositivi di protezione individuale: Eyeshields, Gloves, type N95 (US)