10110434001

Xanthine Oxidase (XOD)

from cow milk

• Sinonimo/i: XOD
• Classificazione EC (Enzyme Commission): 1.1.3.22 (BRENDA, IUBMB)
• Codice UNSPSC: 12352204

Proprietà

• Origine biologica: bovine milk
• Livello qualitativo: 100
• Forma fisica: suspension
• Attività specifica: ~1 units/mg protein (At 25 °C with xanthine as the substrate.)
• Confezionamento: pkg of 1 mL (20 U)
• Produttore/marchio commerciale: Roche
• pH ottimale: 8.5-9.0
• Condizioni di spedizione: wet ice
• Temperatura di conservazione: 2-8°C

Descrizione

Descrizione generale

Xanthine Oxidase (XOD) is a metal flavoprotein. It has FAD, molybdenum and iron in the ratio 2:2:8.

Xanthine:oxygen oxidoreductase

Applicazioni

Xanthine Oxidase (XOD) has been used in the assessment of XOR-mediated NO production from NDHP and assessment of nitrite-derived NO in liver and purified XOR.

Xanthine Oxidase has been used to study tyrosine nitration.

Azioni biochim/fisiol

Xanthine Oxidase (XOD) exhibits a broad substrate specificity including aldehydes, purines and pteridines. Furthermore, this enzyme reduces oxygen to generate superoxide, hydrogen peroxide and reactive oxygen species (ROS). It also reduces nitrite to yield reactive nitrogen species (RNS), such as peroxynitrite and nitric oxide. Owing to its ability to generate RNS and ROS, XOD might play an important role as an antimicrobial agent in the neonatal gut, thereby complementing endogenous enzyme of the intestinal epithelium.

Qualità

Contaminants: <0.005% guanase, NP and uricase, each, <0.05% ADA, <0.05% alkaline phosphatase (4-nitrophenyl phosphate as the substrate)
Note: Chromatographically purified.

Sequenza

XOD is a dimer. Each subunit contains 1 atom of molybdenum, 2 iron-sulfur centers (non-heme iron, ferredoxin-type) and 1 molecule of FAD.

Definizione di unità

One unit (U) xanthine oxidase will produce 1 μmol of uric acid (E_293nm = 12.2 mmol ^-1 x L x cm^-1) from the oxidation of 1 μmol of xanthine in 1 min at 25 °C and pH8.5.

Stato fisico

Suspension in 3.2 M ammonium sulfate solution, 10 mM EDTA, pH approximately 8

Nota sulla preparazione

Activator: O₂
Stabilizers: The substance is stabilized by the addition of EDTA; salicylate is not added.

Altre note

For life science research only. Not for use in diagnostic procedures.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1
• Punto d’infiammabilità (°F): does not flash
• Punto d’infiammabilità (°C): does not flash