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88417

Sigma-Aldrich

MTT Formazan

MTT reduction end product, powder

Sinonimo/i:

Thiazolyl blue formazan

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About This Item

Formula empirica (notazione di Hill):
C18H17N5S
Numero CAS:
Peso molecolare:
335.43
Numero MDL:
Codice UNSPSC:
12352204
ID PubChem:
NACRES:
NA.83

Nome del prodotto

MTT Formazan, MTT reduction end product

Livello qualitativo

Stato

powder

Solubilità

chloroform: 10 mg/mL, clear, dark violet

Stringa SMILE

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
GIFKVQOPAJDJLO-SZVLODPRSA-N

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Categorie correlate

Applicazioni

MTT Formazan has been used in cell viability assay.

Azioni biochim/fisiol

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Altre note

Reviews: Tetrazolium salts and formazans

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)


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