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Key Documents

Altri documenti

M2003

Sigma-Aldrich

MTT Formazan

powder

Sinonimo/i:

Thiazolyl blue formazan

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About This Item

Formula empirica (notazione di Hill):
C18H17N5S
Numero CAS:
57360-69-7
Peso molecolare:
335.43
Numero MDL:
MFCD00080731
Codice UNSPSC:
12171500
ID PubChem:
24896591
NACRES:
NA.47

Saggio

≥97% (TLC)

Forma fisica

powder

Colore

purple

Solubilità

acetone: 1 mg/mL

applicazioni

diagnostic assay manufacturing
hematology
histology

Temperatura di conservazione

room temp

Stringa SMILE

Cc1nc(\N=N\C(=N\Nc2ccccc2)c3ccccc3)sc1C

InChI

1S/C18H17N5S/c1-13-14(2)24-18(19-13)23-22-17(15-9-5-3-6-10-15)21-20-16-11-7-4-8-12-16/h3-12,20H,1-2H3/b21-17+,23-22+
GIFKVQOPAJDJLO-SZVLODPRSA-N

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Applicazioni

MTT Formazan has been used to measure cell viability.

Azioni biochim/fisiol

MTT assay is a colorimetric reaction, generally used to examine cell viability. It involves the enzymatic reduction of a yellow-colored, water-soluble, 3-[4,5-dimethylthiazole-2-yl]-2,5-diphenyltetrazolium bromide (MTT) to purple-colored, water-insoluble, MTT-formazan. This reaction is catalyzed by mitochondrial dehydrogenase and only occurs in viable cells. Thus, the amount of blue formazan is proportional to the number of viable cells. The absorbance of the formazan solution is analyzed spectrophotometrically at 570 nm.

Codice della classe di stoccaggio

11 - Combustible Solids

Classe di pericolosità dell'acqua (WGK)

WGK 3

Punto d’infiammabilità (°F)

Not applicable

Punto d’infiammabilità (°C)

Not applicable

Dispositivi di protezione individuale

Eyeshields, Gloves, type N95 (US)

Certificati d'analisi (COA)

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