13080801

CHO-K1-AC-free

• Codice UNSPSC: 41106514
• NACRES: NA.81

Proprietà

• Origine biologica: hamster ovary
• Descrizione: Hamster Chinese Ovary, serum-free
• Confezionamento: tube of 5 μg 13080801-DNA-5UG; pkg of vial of cells 13080801-1VL
• Modalità di accrescimento: suspension
• Morfologia: Aggregates in suspension
• tecniche: cell culture | mammalian: suitable

Descrizione

Descrizione della linea cellulare

This is a derviative of the CHO-K1 cell line, ECACC catalogue no. 85051005 which has been adapted to grow in the animal component free medium, CHO PF Media (C5467)

Confezionamento

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

Terreno di coltura

CHO PF Media (C5467) + 8mM L-Glutamine (G7513). When freezing cells use freeze medium composed of 50:50 fresh:conditioned medium + 10% DMSO.

Mantenimento delle subcolture

Maintain cultures between approximately 3-9 x 10⁵ cells/ml; 5% CO₂; 37°C. Cells grow as aggregates in suspension. Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 10⁵ cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days.

Altre note

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

Cultures from PHE Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable