Passa al contenuto
Merck
Tutte le immagini(1)

Key Documents

View All Documentation

60983

Millipore

Kovac′s reagent for indoles

suitable for microbiology

Sinonimo/i:

4-(Dimethylamino)benzaldehyde solution, Indole reagent according to Kovac

Autenticatiper visualizzare i prezzi riservati alla tua organizzazione & contrattuali
Tutte le immagini(1)

About This Item

Formula empirica (notazione di Hill):
C9H11NO
Numero CAS:
100-10-7
Peso molecolare:
149.19
Beilstein:
4132845
Numero MDL:
MFCD00003381
Codice UNSPSC:
41171621
ID PubChem:
329759335
NACRES:
NA.85

Durata

limited shelf life, expiry date on the label

Livello qualitativo

100

Composizione

n-butanol
4-dimethylaminobenzaldehyde
hydrochloric acid

tecniche

microbe id | specific enzyme detection: suitable

Indice di rifrazione

n20/D 1.400-1.420

applicazioni

agriculture
clinical testing
environmental
food and beverages
microbiology

Temperatura di conservazione

2-8°C

Compatibilità

Escherichia coli
coliforms

Stringa SMILE

[H]C(=O)c1ccc(cc1)N(C)C

InChI

1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3
BGNGWHSBYQYVRX-UHFFFAOYSA-N

Cerchi prodotti simili? Visita Guida al confronto tra prodotti

Descrizione generale

Kovac′s reagent for indoles is a solution of 4-(dimethylamino)benzaldehyde and hydrochloric acid in n-butanol.

Applicazioni

Kovac′s reagent for indoles has been used for enumerating E. coli O157:H7 in ground beef samples in different scenarios of stored, thawed, and as cooked patty samples. It has also been used for E coli enumeration in dewatered blackwater.

Azioni biochim/fisiol

Kovac′s reagent detects the indole and aids in identifying the indole-positive and indole-negative microorganisms. E. coli can split tryptophan into indole and α-aminopropionic acid in the presence of the enzyme tryptophanase. p-Aminobenzaldehyde present in the reagent reacts with indole to form a cherry-red complex, that is soluble in alcohol, ether, and chloroform. It is recommended to use glucose free media and peptone with a high tryptophan content for the test.

Avvertenze

Danger

Indicazioni di pericolo

H226,H290,H302,H315,H318,H335,H336

Classi di pericolo

Acute Tox. 4 Oral - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Irrit. 2 - STOT SE 3

Organi bersaglio

Central nervous system, Respiratory system

Codice della classe di stoccaggio

3 - Flammable liquids

Classe di pericolosità dell'acqua (WGK)

WGK 1

Punto d’infiammabilità (°F)

96.8 °F

Punto d’infiammabilità (°C)

36 °C

Dispositivi di protezione individuale

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

Elenchi normativi

Forniamo informazioni su eventuali restrizioni prevalentemente per i prodotti chimici. Per altre tipologie di prodotto siamo in grado di fornire soltanto informazioni limitate. Nessuna segnalazione significa che nessuno dei componenti è citato in un elenco. È dovere dell’utilizzatore assicurarsi che il prodotto venga impiegato in maniera sicura e a norme di legge.

EU REACH Annex XVII (Restriction List)

CAS No.

Scegli una delle versioni più recenti:

Certificati d'analisi (COA)

Lot/Batch Number
BCCG3903
BCCD8214
BCCC3021
BCCC3018
BCCB0089

Non trovi la versione di tuo interesse?

Se hai bisogno di una versione specifica, puoi cercare il certificato tramite il numero di lotto.

Cerca un COA

Possiedi già questo prodotto?

I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.

Visita l’Archivio dei documenti

I clienti hanno visto anche

Slide 1 of 10

1 of 10

Millipore

Millipore

07689

O′Meara′s Reagent

4-(Dimethylamino)benzaldehyde puriss. p.a., reag. Ph. Eur., ≥99% (perchloric acid titration)

Sigma-Aldrich

33130

4-(Dimethylamino)benzaldehyde

Methyl Red solution suitable for microbiology

Millipore

08714

Methyl Red solution

Millipore

Millipore

38497

Nitrate Reagent A

4-(Dimethylamino)benzaldehyde suitable for histochemical demonstration of nitro blue tetrazolium reduction in neutrophils

Sigma-Aldrich

D2004

4-(Dimethylamino)benzaldehyde

4-(Dimethylamino)benzaldehyde for synthesis

Sigma-Aldrich

8.03057

4-(Dimethylamino)benzaldehyde

TDA Reagent suitable for microbiology

Millipore

80353

TDA Reagent

Indole Assay Kit sufficient for 100 colorimetric tests

Sigma-Aldrich

MAK326

Indole Assay Kit

Nitrate Reagent B suitable for microbiology

Millipore

39441

Nitrate Reagent B

MR-VP broth Methyl-red VOGES-PROSKAUER broth, suitable for microbiology

Millipore

1.05712

MR-VP broth

Stavros G Manios et al.
Meat science, 101, 25-32 (2014-12-03)
The effect of common handling practices (i.e., freezing, thawing and cooking) of beef patties on the survival of Salmonella spp. and Escherichia coli O157:H7, was evaluated. Inoculated ground beef was stored at -22 °C for 5 and 75 days. After
Ragnhildur Gunnarsdóttir et al.
Environmental science & technology, 46(22), 12408-12416 (2012-11-02)
Wastewater treatment in many Arctic regions is inadequate, even nonexisting. Natural freezing of wastewater in those areas may be beneficial for reduction of microorganisms. The aim of this study was to investigate the effect of long-term freezing, and repeated freezing
Jun-Min Guo et al.
Journal of agricultural and food chemistry, 58(11), 6556-6561 (2010-05-15)
A simple colorimetric method for the differentiation of indoleacetic acid (IAA) and indolebutyric acid (IBA) in plant samples is described. The color change is based upon the reaction between the auxins and p-(dimethylamino)benzaldehyde (PDAB, Ehrlich reagent) following the electrophilic substitution
G P Kushto et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 54A(6), 799-819 (1998-07-17)
Results of a normal coordinate analysis based on infrared and Raman spectra of six isotopic forms of 4-(dimethylamino)benzaldehyde are reported (DABA, DABA-CDO, DABA-13CDO, DABA-13CHO, DABA-CHO18O, and DABA-3,5-D). Cs point group symmetry has been applied and all motions except the internal
N T Yatsimirskaya et al.
Analytical biochemistry, 229(2), 249-255 (1995-08-10)
Yields of colored Schiff base products of the reversible condensation of 6-aminopenicillanic and 7-aminocephalosporanic acids with para-dimethylaminobenzaldehyde in a weakly acid aqueous solution strongly increase in the presence of micelles of sodium dodecyl sulfate. The binding constants of reactants and
Visualizza tutti i documenti correlati

Articoli

Clostridium perfringens, their Properties and their Detection

An article regarding the Role of Clostridium perfringens and their detection, identification, and differentiation from Sigma-Aldrich.com

Identification of Microorganisms Based on Color

For microbiologists the most fundamental stain was developed in 1884 by the Danish bacteriologist Hans Christian Gram.

Differentiation of Escherichia coli from coliforms

Sigma-Aldrich.com presents an article concerning Differentiation of Escherichia coli from coliforms.

Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

Contatta l'Assistenza Tecnica.