67309

Kovac′s reagent for indoles

suitable for microbiology

• Sinonimo/i: 4-(Dimethylamino)benzaldehyde solution
• Formula empirica (notazione di Hill): C₉H₁₁NO
• Numero CAS: 100-10-7
• Peso molecolare: 149.19
• Beilstein: 4132845
• Numero MDL: MFCD00003381
• Codice UNSPSC: 41171621
• ID PubChem: 329760541
• NACRES: NA.85

Proprietà

• agenzia: according to ISO 16654:2001
• Livello qualitativo: 200
• Nome Commerciale: BioChemika
• Durata: limited shelf life, expiry date on the label
• Composizione: 4-(dimethlyamino)benzaldehyde, 50 g/L ; hydrochloric acid, 240 g/L ; isoamylic alcohol, 710 g/L
• tecniche: microbe id | specific enzyme detection: suitable
• applicazioni: agriculture; clinical testing; environmental; food and beverages; microbiology
• Temperatura di conservazione: 2-8°C
• Compatibilità: Escherichia coli; coliforms
• Stringa SMILE: [H]C(=O)c1ccc(cc1)N(C)C
• InChI: 1S/C9H11NO/c1-10(2)9-5-3-8(7-11)4-6-9/h3-7H,1-2H3; BGNGWHSBYQYVRX-UHFFFAOYSA-N

Descrizione

Descrizione generale

Kovac′s reagent is prepared by mixing p-dimethylaminobenzaldehyde, isoamyl alcohol and concentrated hydrochloric acid. For identification of an organism, the formation of Indole from a tryptophan substrate is a useful diagnostic tool. Indole production is a crucial test in identification of Escherichia coli. After incubation, adding the reagent will help in determination if Indole that has been produced. The reagent turns red when reacts with Indole.

Applicazioni

In the presence of oxygen, some bacteria, like E.coli, are able to split tryptophan into indole and alpha-aminopropionic acid. This reagent is for detecting the indole and identify the indole-positive and indole-negative microorganisms.

Kovac′s reagent may be used for spot test for determination of indole by saturating filter paper with the reagent. It may also be used for initial spot test for determination of indole followed by quantification by spectrophotometric assay and HPLC-UV/Vis-MS/MS methods.

Informazioni sulla sicurezza

• Pittogrammi: GHS02,GHS05,GHS07
• Avvertenze: Danger
• Indicazioni di pericolo: H226,H290,H314,H317,H332,H335
• Consigli di prudenza: P210 - P233 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Classi di pericolo: Acute Tox. 4 Inhalation - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 3
• Organi bersaglio: Respiratory system
• Codice della classe di stoccaggio: 3 - Flammable liquids
• Classe di pericolosità dell'acqua (WGK): WGK 3
• Punto d’infiammabilità (°F): 109.4 °F
• Punto d’infiammabilità (°C): 43 °C
• Dispositivi di protezione individuale: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter