MABT336

Anti-TKS5 Antibody, clone 13H6.3

clone 13H6.3, from mouse

• Sinonimo/i: Adapter protein TKS5, Five SH3 domain-containing protein, SH3 multiple domains protein 1, Tyrosine kinase substrate with five SH3 domains, SH3 and PX domain-containing protein 2A
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: 13H6.3, monoclonal
• Reattività contro le specie: human
• Confezionamento: antibody small pack of 25 μg
• tecniche: immunohistochemistry: suitable (paraffin); western blot: suitable
• Isotipo: IgG1κ
• N° accesso NCBI: NP_055446
• N° accesso UniProt: Q5TCZ1
• Condizioni di spedizione: ambient
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... SH3PXD2A(9644)

Descrizione

Descrizione generale

SH3 and PX domain-containing protein 2A (UniProt: Q5TCZ1; also known as Adapter protein TKS5, Five SH3 domain-containing protein, SH3 multiple domains protein 1, Tyrosine kinase substrate with five SH3 domains) is encoded by the SH3PXD2A (also known as FISH, KIAA0418, SH3MD1, TKS5) gene (Gene ID: 9644) in human. Adapter protein TKS5 is a scaffolding protein that is required for podosome formation, degradation of the extracellular matrix, and for invasion in some cancer cells. Three isoforms of TKS5 have been described that are produced by alternative splicing. TKS5 is a cytoplasmic protein in normal cells, but it localizes to podosomes in SRC-transformed cells. It is found in several cancer cell lines, particularly invasive breast carcinomas and melanomas. TKS5 contains one PX (phx homology) domain and five SH3 domains. The PX domain is required for podosome localization because of its ability to bind phosphoinosiltols, such as PtdIns3P and PtdIns(3,4)P2 and to lesser extent PtdIns5P and PtdIns(3,5)P2. Its binding with ADAM12, ADAM15 and ADAM19 is mediated by the fifth SH3 domain. The intramolecular interaction of the PX domain with the third SH3 domain maintains the protein in the cytoplasm and phosphorylation disrupts this interaction, resulting in the redistribution of the protein from cytoplasm to the perimembrane region.

Specificità

Clone 13H6.3 detects SH3 and PX domain-containing protein 2A (adapter protein TKS5) in human cells. It targets and epitope with in 60 amino acids crom the C-terminal half.

Immunogeno

GST/His-tagged recombinant fragment corresponding to 60 amino acids from the C-terminal half of human SH3 and PX domain-containing protein 2A (Adapter protein TKS5).

Applicazioni

Anti-TKS5, clone 13H6.3, Cat. No. MABT336, ia a mouse monoclonal antibody that detects TKS5 and has been tested for use in Immunohistochemistry (Paraffin) and Western Blotting.

Immunohistochemistry Analysis: A 1:50 dilution from a representative lot detected TKS5 in human breast cancer tissue.

Research Category
Cell Structure

Qualità

Evaluated by Western Blotting in SCC61 and SK-MEL28 cell lysates.

Western Blotting Analysis: 0.5 µg/mL of this antibody detected TKS5 in 10 µg of SCC61 and SK-MEL28 cell lysates.

Descrizione del bersaglio

~160 kDa observed; 125.29 kda calculated. Uncharacterized bands may be observed in some lysate(s).

Stato fisico

Format: Purified

Protein G purified

Purified mouse monoclonal antibody IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Stoccaggio e stabilità

Stable for 1 year at 2-8°C from date of receipt.

Altre note

Concentration: Please refer to lot specific datasheet.

Esclusione di responsabilità

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 12 - Non Combustible Liquids
• Classe di pericolosità dell'acqua (WGK): WGK 1