MAB1622
Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6
clone AA6, Chemicon®, from mouse
Sinonimo/i:
(ALPHA)II-SPECTRIN, Alpha-II spectrin, Fodrin alpha chain, Spectrin, non-erythroid alpha chain, non-erythrocytic spectrin alpha, spectrin, alpha, non-erythrocytic 1 (alpha-fodrin), Fodrin α
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About This Item
Codice UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Prodotti consigliati
Origine biologica
mouse
Livello qualitativo
Forma dell’anticorpo
purified immunoglobulin
Tipo di anticorpo
primary antibodies
Clone
AA6, monoclonal
Reattività contro le specie
pig, guinea pig, rabbit, mouse, chicken, rat, human
Reattività contro le specie (prevista in base all’omologia)
mammals
Produttore/marchio commerciale
Chemicon®
tecniche
ChIP: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG1
Compatibilità
not suitable for immunohistochemistry (Paraffin)
N° accesso NCBI
N° accesso UniProt
Condizioni di spedizione
wet ice
modifica post-traduzionali bersaglio
unmodified
Informazioni sul gene
human ... SPTAN1(6709)
Descrizione generale
Non-erythroid Spectrin, also known as Fodrin, plays a role in stabilizing membrane structures, maintaining cell shape and linking the cytoskeleton to plasma membranes or intracellular vesicles. As one of the primary targets cleaved by activated caspases during apoptosis, the full-length ~240 kDa protein can be cleaved to yield an N-terminal product of ~150 kDa, and C-terminal products of ~120 and ~35 kDa.
Specificità
Specific for the 240, 150, and 120 kDa forms of alpha-spectrin/alpha-fodrin molecule of all mammalian nonerythroid cells and chicken alpha-spectrin.
Wide-mammalian reactivity is expected.
Immunogeno
Chicken red blood cell membranes purified by hypotonic lysis and mechanical enucleation.
Applicazioni
Immunohistochemistry (IFA):
A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry.
Does not work on paraffin sections.
Optimal working dilutions must be determined by end user.
Chromatin Immunopreciptiation:
A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908).
A 1:200-1:400 dilution of a previous lot was used in immunohistochemistry.
Does not work on paraffin sections.
Optimal working dilutions must be determined by end user.
Chromatin Immunopreciptiation:
A previous lot of this antibody was used to immunoprecipitate Spectrin Alpha from chromatin-associated protein extracts derived from normal human, HeLa, FA-A, and transduced FA-A cells (McMahon, L.W., et al. (1999). J. Biological Chem. 274:32904-32908).
Anti-Spectrin alpha chain (nonerythroid) Antibody, clone AA6 is an antibody against Spectrin alpha chain (nonerythroid) for use in IH, IP, WB, ChIP.
Research Category
Cell Structure
Cell Structure
Research Sub Category
Cytoskeleton
Cytoskeleton
Qualità
Evaluated by Western Blot on staurosporine-treated HeLa lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates.
Western Blot Analysis:
1:500 dilution of this antibody detected N-, C- terminal Spectrin alpha/Fodrin alpha on 10 μg of staurosporine treated HeLa lysates.
Descrizione del bersaglio
~240 kDa full length product, ~150 kDa N-terminal cleaved product and ~120 kDa C-terminal product may be seen in varing amounts in lysates, depending on condition. The smaller sizes represent break down products seen during apoptosis.
Stato fisico
Format: Purified
Protein A purified
Purified mouse monoclonal IgG1 in buffer containing PBS with 1% BSA and 0.09% sodium azide.
Stoccaggio e stabilità
Stable for 12 months at 2-8°C from date of receipt.
Risultati analitici
Control
HeLa cells treated with staurosporine
HeLa cells treated with staurosporine
Altre note
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Note legali
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Esclusione di responsabilità
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Raccomandato
N° Catalogo
Descrizione
Determinazione del prezzo
Codice della classe di stoccaggio
12 - Non Combustible Liquids
Classe di pericolosità dell'acqua (WGK)
WGK 2
Punto d’infiammabilità (°F)
Not applicable
Punto d’infiammabilità (°C)
Not applicable
Certificati d'analisi (COA)
Cerca il Certificati d'analisi (COA) digitando il numero di lotto/batch corrispondente. I numeri di lotto o di batch sono stampati sull'etichetta dei prodotti dopo la parola ‘Lotto’ o ‘Batch’.
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I documenti relativi ai prodotti acquistati recentemente sono disponibili nell’Archivio dei documenti.
T L Brown et al.
The Journal of biological chemistry, 274(33), 23256-23262 (1999-08-07)
Transforming growth factor beta (TGF-beta) is a potent growth inhibitor and inducer of cell death in B-lymphocytes and is essential for immune regulation and maintenance of self-tolerance. In this report the mouse immature B cell line, WEHI 231, was used
C Ayala-Grosso et al.
Neuroscience, 126(4), 927-940 (2004-06-23)
The ability of fimbria-fornix bilateral axotomy to elicit calpain and caspase-3 activation in the rat septohippocampal pathway was determined using antibodies that selectively recognize either calpain- or caspase-cleaved products of the cytoskeletal protein alphaII-spectrin. Radioenzymatically determined choline acetyl transferase (ChAT)
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Siman, R; Zhang, C; Roberts, VL; Pitts-Kiefer, A; Neumar, RW
Journal of Cerebral Blood Flow and Metabolism null
Calpain mediates proteolysis of the voltage-gated sodium channel alpha-subunit.
CR von Reyn, JM Spaethling, MN Mesfin, M Ma, RW Neumar, DH Smith, R Siman, DF Meaney
The Journal of Neuroscience null
Estradiol attenuates programmed cell death after stroke-like injury
Rau, S. W. et al.
The Journal of Neuroscience, 23(36), 11420-11426 (2003)
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