MAB1501-AF647

Anti-Actin Antibody, clone C4, Alexa Fluor^™ 647 conjugate

clone C4, from mouse, ALEXA FLUOR^™ 647

• Sinonimo/i: Actin, alpha skeletal muscle, Alpha-actin-1, Actin
• Codice UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Proprietà

• Origine biologica: mouse
• Livello qualitativo: 100
• Coniugato: ALEXA FLUOR^™ 647
• Forma dell’anticorpo: purified antibody
• Tipo di anticorpo: primary antibodies
• Clone: C4, monoclonal
• Reattività contro le specie: human
• Reattività contro le specie (prevista in base all’omologia): chicken (based on 100% sequence homology)
• tecniche: immunocytochemistry: suitable
• Isotipo: IgG2bκ
• N° accesso NCBI: NP_001026234.1
• N° accesso UniProt: P68139
• Condizioni di spedizione: wet ice
• modifica post-traduzionali bersaglio: unmodified
• Informazioni sul gene: human ... ACTA1(58)

Descrizione

Descrizione generale

Actins are ubiquitous eukaryotic proteins that serve as a multi-functional, basic building blocks of cytoskeletal microfilaments. They play critical roles in a wide range of cellular processes, including cell division, cell migration, chromatin remodeling, trascriptional regulation and vesicle trafficking. These funstions are attributed to their ability to form filaments, which can quickly assemble and disassemble depending upon the needs of the cell. At least six different actin types have been reported in mammals. Although actins show about 90% overall sequence homology, isoforms do not show spatial, temporal and tissue-specific expression patterns and only 50-60% homology is found in their 18 N-terminal residues. Beta and gamma-actins, also known as cytoplasmic actins, are highly conserved in higher animals and are predominantly expressed in non-muscle cells where they control cell structure. Exocytosis, and motility. They are nearly identical proteins and differ only in four amino acids at the N-terminal region. The other four actin isoforms are typically found in specific adult muscle tissue types. Alpha-cardiac and alpha-skeletal actins are expressed in striated cardiac and skeletal muscles, respectively. Alpha and gamma actins are primarily found in vascular smooth muscle and enteric smooth muscles, respectively. It has been shown that under calcium-bound conditions, beta-actin exhibits more dynamic behavior than gamma-actin with higher rates of polymerization and depolymerization. Also, beta- and gamma-actins can readily copolymerize, and the resulting filaments exhibit polymerization and depolymerization rates that vary depending on the ration of beta- to gamma-actin (Lessard, JL.,et al.(1988). Cell Motility Cytoskeleton 10(3); 349-362.

Specificità

To date, all animal species and cell types with an actin form react by indirect immunofluorescence or immunoblot, including plant actin.

Applicazioni

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected Actin in NIH/3T3 cells.
The unconjugated version of this antibody (Cat. No. MAB1501) has been demonstrated to perform in WB, ELISA, ICC, IF, IHC, IH(P).

This Anti-Actin Antibody, clone C4, Alexa Fluor^™ 647 conjugate is validated for use in Immunocytochemistry for the detection of Actin.

Qualità

Evaluated by Immunocytochemistry in HeLa cells.

Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected Actin in HeLa cells.

Descrizione del bersaglio

40 kDa observed

Altre note

Concentration: Please refer to lot specific datasheet.

Note legali

ALEXA FLUOR is a trademark of Life Technologies

Informazioni sulla sicurezza

• Codice della classe di stoccaggio: 10 - Combustible liquids
• Classe di pericolosità dell'acqua (WGK): WGK 2
• Punto d’infiammabilità (°F): Not applicable
• Punto d’infiammabilità (°C): Not applicable