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Key Documents

Z722553

ThermalSeal RT2RR™ film

non-sterile, sealing films for real-time qPCR

别名:

ThermalSeal RT™ film, real-time qPCR plate seal

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About This Item

分類程式碼代碼:
41122410
NACRES:
NB.22

材料

polyester film

無菌

non-sterile

包裝

pack of 100 ea

製造商／商標名

Excel Scientific TS-RT2RR-100

技術

PCR: suitable
qPCR: suitable

長度

130.6 mm

寬度 × 長度

7.78 cm × 13.08 cm

寬度

77.8 mm

顏色

clear

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相关类别

一般說明

A combination of optically transparent polyester film with a strong, ultra-smooth, non-absorbing, non-fluorescing medical-grade adhesive for superior performance in real-time PCR applications. In place of the customary paper backing, ThermalSeal RT has a plastic liner which is easily removed before use and contributes smoothness and extreme optical clarity to the adhesive. Dimensions are 3.125 x 5.625 in. to seal PCR plates. Length between the perforated ends is 4.8 in.
  • Ultra-high optical clarity
  • Heat resistant, recommended for temperatures from -40°C to +120°C
  • Certified DNase, RNase, and nucleic acid free
ThermalSeal RT2RR™ 50 μm polyester sealing films for real-time PCR are sized to fit within the edges of raised-rim 96 well plates. The same consistent ultra-high optical clarity as Excel′s ThermalSeal RT™ sealing films makes possible more reproducible, reliable, and consistent DNA amplification measurements. An inert, strong, temperature-resistant adhesive assures reliable sealing around each well. Two end tabs assist in positioning the sealing film. Easy removal of the end tabs at perforated boundaries prevents lifting and higher evaporation rates that can occur with sealing films that overlap the plate rim. Recommended for specific DNA sequence detection in clinical diagnostics, forensic science and basic research.
Dimensions 77.8 by 130.8 mm. With end tabs removed, length is 118.1 mm with 45° corners.
  • Ultra-high optical clarity
  • Fit within plate rim to prevent evaporation due to sealing film lifting
  • Heat resistant, recommended for temperatures from
  • -40 °C to +120 °C
  • Certified DNase, RNase, and Nucleic Acid free

法律資訊

ThermalSeal is a registered trademark of Excel Scientific, Inc.
ThermalSeal RT is a trademark of Excel Scientific, Inc.
ThermalSeal RT2RR is a trademark of Excel Scientific, Inc.

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实验方案

qPCR Gene Expression Using Probe Detection

The most common application for qPCR is the measurement of a gene transcript or copy number quantity relative to one or more reference genes using probe detection.

Primer Concentration Optimization Protocol

Primer Concentration Optimization Protocol is an approach to create a matrix of reactions. This is used to test a range of concentrations for each primer against different concentrations of the partner primer.

Multiplex qPCR Protocol

A protocol that can be used as a basic template for qPCR incorporating up to four detection probes. In these reactions, primers and probe are included at a final concentration of 200 nM and are run using LuminoCt ReadyMix.

Standard Reverse Transcription Protocol (Two-step)

Reverse transcription is the analysis of gene expression to measure concentration mrna a gene. there are several challenges such analyses, as differences in halflife between different transcripts, temporal patterns and lack correlation protein.

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